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Book Cover
Author Yuryev, Anton

Title PCR primer design / edited by Anton Yuryev
Published Totowa, N.J. : Humana Press, 2007


Description 1 online resource (xiii, 431 pages) : illustrations
Series Methods in molecular biology ; 402
Methods in molecular biology (Clifton, N.J.) ; v. 402
Contents Physical principles and visual-OMP software for optimal PCR design / J. SantaLucia, Jr. -- OLIGO 7 primer analysis software / W. Rychlik -- Selection for 3'-end triplets for polymerase chain reaction primers / K. Onodera -- The reference point method in primer design / T. Kampke -- PCR primer design using statistical modeling / A. Yuryev -- Developing a statistical model for primer design / J. Huang and A. Yuryev -- Gst-prime: An algorithm for genome-wide primer design / D. Leister and C. Varotto -- Genome-scale probe and primer design with primegens / G.P. Srivastava and D. Xu -- SNPbox: Web-based high-throughput primer design with an eye for repetitive sequences / S. Weckx [and others] -- Fast masking of repeated primer binding sites in eukaryotic genomes / R. Andreson, L. Kaplinski and M. Remm -- Degenerate primer design: Theoretical analysis and the hyden program / C. Linhart and R. Shamir -- An iterative method for selecting degenerate multiplex PCR primers / R. Souvenir [and others] -- Primer design for multiplexed genotyping / L. Kaderali -- Multiplx: Automatic grouping and evaluation of PCR primers / L. Kaplinski and M. Remm -- Multiprimer: A system for microarray PCR primer design / R. Fernandes and S. Skiena -- Modified oligonucleotides as tools for allele-specific amplification / M. Strerath [and others] -- AlleleID: A pathogen detection and identification system / A. Apte and S. Singh -- Designing primers for whole genome PCR scanning using the software package GenoFrag: A software package for the design of primers dedicated to whole-genome scanning by LR-PCR / N. Ben Zakour and Y. Le Loir -- Designing PCR primer for DNA methylation mapping / L.C. Li -- Bisearch: EPCR tool for native or bisulfite-treated genomic template / T. Aranyi and G.E. Tusnady -- Graphical design of primers with perlprimer / O. Marshall -- GST-PRIME: an algorithm for genome-wide primer design / D. Leister and C. Varotto -- Genome-scale probe and primer design with PRIMEGENS / G.P. Srivastava and D. Xu
Summary In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists - geneticists, molecular biologists, molecular geneticists, and more - who routinely use PCR in their research
Notes Title from title screen (viewed November 20, 2007)
Bibliography Includes bibliographical references and index
Notes English
Subject Polymerase chain reaction.
DNA polymerases.
DNA probes.
Mathematical models.
Genetics -- Technique.
Nucleic acid probes.
Nucleic acids.
Molecular probes.
Public health.
Medical care.
DNA Primers
Polymerase Chain Reaction
Models, Statistical
Sequence Analysis, DNA
Statistics as Topic
Sequence Analysis
Nucleic Acid Amplification Techniques
DNA Probes
Models, Theoretical
Genetic Techniques
Epidemiologic Methods
Health Care Evaluation Mechanisms
Nucleic Acid Probes
Investigative Techniques
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Molecular Probes
Quality of Health Care
Public Health
Laboratory Chemicals
Health Care Quality, Access, and Evaluation
Environment and Public Health
Delivery of Health Care
Nucleic Acids, Nucleotides, and Nucleosides
Specialty Uses of Chemicals
Chemical Actions and Uses
Chemicals and Drugs
Patient Care
mathematical models.
public health.
Polymerase chain reaction.
DNA polymerases.
Public health
Nucleic acids
Nucleic acid probes
Molecular probes
Medical care
Mathematical models
Genetics -- Technique
DNA probes
DNA polymerases
Polymerase chain reaction
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
ISBN 9781588297259