Title Protocols for oligonucleotide conjugates : synthesis and analytical techniques / edited by Sudhir Agrawal ; foreword by H. Gobind Khorana

Published Totowa, N.J. : Humana Press, ©1994

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Description 1 online resource (xiv, 377 pages) : illustrations Series Methods in molecular biology ; v. 26 Methods in molecular biology (Clifton, N.J.) ; v. 26 Contents Protecting groups in oligonucleotide synthesis / Etienne Sonveaux -- Incorporation of modified bases into oligonucleotides / Rich B. Meyer -- Functionalization of oligonucleotides with amino groups and attachment of amino specific reporter groups / Sudhir Agrawal -- Functionalization of oligonucleotides by the incorporation of thio-specific reporter groups / Jacqueline A Fidanza, Hiroaki Ozaki, Larry W. McLaughlin -- Postsynthesis functionalization of oligonucleotides / Barbara C. Chu, Leslie E. Orgel -- Oligonucleotide-enzyme conjugates / Jerry L Ruth -- Oligonucleotides containing degenerate bases: synthesis and uses / Paul Kong Lin, Daniel M. Brown -- Synthesis of ¹⁵N-labeled DNA fragments / Roger A Jones -- Analysis and purification of synthetic oligonucleotides by high-performance liquid chromatography / William J. Warren, George Vella -- Sequence analysis of oligodeoxyribonucleotides / Diane M. Black, Peter T. Gilham -- Gel-capillary electrophoresis analysis of oligonucleotides / Alex Andrus -- Nuclear magnetic resonance studies of oligonucleotides / Jerzy W. Jaroszewski, Siddhartha Roy, Jack S. Cohen -- Mass spectrometry of nucleotides and oligonucleotides / Thomas D. McClure, Karl H. Schram -- Extracting thermodynamic data from equilibrium melting curves for oligonucleotide order-disorder transitions / Kenneth J. Breslauer Summary You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay Analysis oligonucleotiden oligonucleotides derivaten derivatives synthese synthesis analytische methoden analytical methods Biochemistry Biochemie Notes Source of title; title from title screen Bibliography Includes bibliographical references and index Notes English Subject Oligonucleotides -- Synthesis -- Laboratory manuals Oligonucleotides -- Analysis -- Laboratory manuals Oligonucleotides -- chemistry Oligonucleotides -- Analysis Oligonucleotides -- Synthesis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Agrawal, Sudhir. LC no. 93023127 ISBN 9781592595136 1592595138 0896032523 9780896032521

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