Title Oligonucleotide synthesis : methods and applications / edited by Piet Herdewijn

Published Totowa, N.J. : Humana Press, ©2005

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Description 1 online resource (xvi, 435 pages) : illustrations Series Methods in molecular biology ; 288 Methods in molecular biology (Clifton, N.J.) ; v. 288 Contents Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger [and others] -- RNA synthesis using 2'-o-(tert-butyldimethylsilyl) protection / Brian S. Sproat -- RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / S. Hartsel [and others] -- DTD; new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis -- Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi -- Assay for evaluating ribonuclease H-mediated degradation of RNA/antisense oligonucleotide duplexes / Annie Galarneau [and others] -- Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg -- Solid-phase chemistry of circular oligonucleotides / Enrique Pedroso [and others] -- LNA synthesis / Henrik M. Pfundheller [and others] -- Synthesis of DNA mimics representing HypNa-pPNA heterooligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva -- Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela [and others] -- Introduction of hypermodified nucleotides in rna / Darell Davis and Ashok C. Bajji -- Chemical methods for peptide/oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait -- Biotin labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho [and others] -- Universal labeling chemistry for nucleic acid detection on DNA-chips -- Ali Laayoun, Eloy [and others] -- Post-synthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun -- Fluorescence based online detection as an analytical tool in RNA electrophoresis / Ute Scheffer and michael göbel -- Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras -- High thoughput production of optimized primers (fimers) for whole genome direct sequencing / Nikolai Polushin [and others] -- Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel -- DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly, Jérome Kluza, Christopher Martin / Thomas Ellis, and Michael J. Waring -- Gene targeting using PNA (peptide nucleic acid) / Peter E. Nielsen -- Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau -- In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke -- In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse [and others] -- Short double-stranded RNA (sirna) as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy Summary Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today Bibliography Includes bibliographical references and index Notes English Print version record Subject Oligonucleotides -- Synthesis -- Laboratory manuals Oligonucleotides -- chemical synthesis Oligonucleotides -- chemistry SCIENCE -- Life Sciences -- Molecular Biology. Oligonucleotides -- Synthesis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Herdewijn, Piet LC no. 2004002114 ISBN 1588292339 9781588292339 1592598234 9781592598236 1280359854 9781280359859 9786610359851 6610359857

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