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Title Mouse oocyte development : methods and protocols / edited by Marie-Hélène Verlhac, Marie-Emilie Terret
Published New York, NY : Humana Press, [2018]
©2018

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Description 1 online resource (xi, 174 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1818
Methods in molecular biology (Clifton, N.J.) ; v. 1818. 1064-3745
Contents Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse -- In vitro Culture of Mouse Oocytes for Meiotic Maturation -- In vitro Maturation and Culture of Human Oocytes -- Pronuclear Transfer in Human Oocytes -- In vitro Fertilization of Mouse Oocyte -- Profiling Maternal mRNA Translation during Oocyte Development -- Transcriptome Profiling of Single Mouse Oocytes -- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation -- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation -- Triple-colour Live Imaging of Mouse Oocytes -- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes -- Manipulating Cohesin Levels in Live Mouse Oocytes -- Optogenetic Manipulation of Mouse Oocytes -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension
Summary This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed July 2, 2018)
Subject Mice -- Reproduction -- Laboratory manuals
Ovum -- Laboratory manuals
Mice -- embryology
Oocytes -- growth & development
In Vitro Oocyte Maturation Techniques
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Mice -- Reproduction
Ovum
Form Electronic book
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Author Verlhac, Marie-Hélène, editor
Terret, Marie-Emilie, editor
ISBN 9781493986033
1493986031