| Description |
1 online resource (xvi, 480 pages) : illustrations (some color) |
| Series |
Springer protocols |
|
Methods in molecular biology, 1940-6029 ; 610 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 610
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| Contents |
Sec. 1. Reactive oxygen and nitrogen species -- 1. In vivo imaging of free radicals and oxygen / Deepti S. Vikram, Brian K. Rivera, and Periannan Kuppusamy -- 2. In vivo measurement of tissue oxygen using electron paramagnetic resonance spectroscopy with oxygen-sensitive paramagnetic particle, lithium phthalocyanine / F. Hyodo [and others] -- 3. Measurement of plasma nitrite by chemiluminescence / Enika Nagababu and Joseph M. Rifking -- 4. Determination of glutathione, mitochondrial transmembrane potentital, and cytotoxicity in H9c2 cardiomyoblasts exposed to reactive oxygen and nitrogen species / K. Sathishkumar [and others] -- Sec. 2 Natural and synthetic antioxidants -- 5. Phenolic acids and flavonoids: occurrence and analytical methods / Constantine D. Stalikas -- 6. Design, synthesis and action of antiatherogenic antioxidants / Osamu Cynshi, Kunio Tamura, Etsuo Niki -- 7. Preparation of drug-loaded polymeric nanoparticles and evaluation of the antioxidant activity against lipid peroxidation / Adriana R. Pohlmann [and others] -- 8. Nanoparticle and iron chelators as a potential novel alzheimer therapy / Gang Liu [and others] -- 9. Synthesis and characterization of polymer nanocarriers fro the targeted delivery of therapeutic enzymes / Eric Simone [and others] -- 10. Assessment of antioxidant activity of eugenol in vitro and in vivo / Enika Nagababu [and others] -- Sec. 3. Cellular oxidative stress -- 11. Generation of stable oxidative stress-resistant phenotypes in Chinese hamster fibroblasts chronically exposed to hydrogen peroxide or hyperoxia / Douglas R. Spitz and Shannon J. Sullivan -- 12. A simple method for effective and safe removal of membrane cholesterol from lipid rafts in vascular endothelial cells / Michelle A. Kline [and others] -- 13. Superoxide dismutase, a target for gene therapeutic approach to reduce oxidative stress in erectile dysfunction / W. Deng [and others] -- 14. Assessing the reductive capacity of cells by measuring the recycling of ascorbic and lipoic acids / James M. May -- 15. Biomarkers of oxidative stress / Muthuswamy Balasubramanyam [and others] -- 16. Simultaneous analysis of expression of multiple redox-sensitive and apoptotic genes in hypothalmic neurons exposed to cholesterol secoaldehyde / K. Sathishkumar [and others] -- 17. Redox homeostatis and cellular stress response in aging and neurodegeneration / Vittorio Calabrese [and others] -- 18. Gene therapy techniques for the delivery of endothelial nitric oxide synthase to the lung for pulmonary hypertension / W. Deng [and others] -- Sec. 4. DNA oxidation, oxidative lipidomics and biomarkers -- 19. A General method for quantifying sequence effects on nucleobase oxidation in DNA / Yelena Margolin and Peter C. Dedon -- 20. Analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine by liquid chromatography-tandem mass spectrometry / Mark D. Evans [and others] -- 21. Oxidative lipidomics of apoptosis / Vladimir A. Tyurine [and others] -- 22. Simultaneous analysis of multiple lipid oxidation products in vivo by liquid chromatographic-mass spectrometry (LC-MS) / Huiyong Yin, Todd Davis, Ned A. Porter -- 23. Lipoxygenase-catalyzed phospholipid peroxidation / E. Susan O'Connor Butler [and others] -- 24. Oxidized low-density lipoprotein / Sampath Parthasarathy [and others] -- 25. Detection and localization of markers of oxidative stress by in situ methods / Paula I. Moreira [and others] -- 26. Enzyme immunoassay of isoprostanes / Denis M. Callewaert, Charles Sloan -- 27. Application of membrane extraction with sorbent interface with breath analysis / Victor Ma [and others] |
| Summary |
In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology"!series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike |
| Analysis |
vrije radicalen |
|
free radicals |
|
antioxidatieve eigenschappen |
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antioxidant properties |
|
Biochemistry |
|
Biochemie |
| Notes |
Title from PDF title page (SpringerLink, viewed January 6, 2010) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Free radicals (Chemistry) -- Pathophysiology
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Antioxidants.
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Oxidation, Physiological.
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Free radicals (Chemistry)
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Antioxidants -- therapeutic use
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Free Radicals -- adverse effects
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Oxidative Stress -- physiology
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Reactive Nitrogen Species -- adverse effects
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Reactive Oxygen Species -- adverse effects
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Antioxidants
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Free Radicals
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antioxidant.
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Antioxidants
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Free radicals (Chemistry)
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Free radicals (Chemistry) -- Pathophysiology
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Oxidation, Physiological
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Uppu, Rao M.
|
| ISBN |
9781603270298 |
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1603270299 |
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9781607614913 |
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160761491X |
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9781617796838 |
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1617796832 |
|
