Title A practical guide to frozen section technique / Stephen R. Peters, editor

Published New York : Springer, ©2010

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Description 1 online resource (xi, 193 pages) : illustrations (chiefly color) Contents Understanding and Maintaining the Cryostat / Philip Hyam -- Gross Examination of Tissues in the Frozen Section Room / Stephen R. Peters -- Embedding of Tissue for Frozen Section / Stephen R. Peters -- Cutting the Frozen Section / Stephen R. Peters -- Variables Affecting the Cutting Properties of Tissues and the Resulting Artifacts / Stephen R. Peters -- Fixation, Staining and Coverslipping of Frozen Section Slides / Stephen R. Peters and Catherine Susan Delia -- Microscopic Interpretation in the Frozen Section Setting / Stephen R. Peters -- Frozen Section Techniques Used in Mohs Micrographic Surgery / Barbara Beck and Stephen R. Peters -- Frozen Section Technique in the Animal Research Setting / Charles W. Scouten Summary A Practical Guide to Frozen Section Technique offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step by step approach to learning continuous motion brush technique. Emphasis is placed on recognizing and correcting artifacts during the preparation process. The book addresses all of the steps in the preparation of slides from cutting through cover-slipping. The author's unique, original techniques for tissue embedding including face down embedding in steel well bars, frozen block cryoembedding and paper cryoembedding are detailed. Variables key to the quality of the preparation including block temperature, tissue properties and section thickness are detailed. The book also covers understanding the cryostat and basic maintenance and care. Sections covering techniques used in Mohs dermatologic surgery, and techniques used in basic animal and human research are discussed by noted experts in their field. A Practical Guide to Frozen Section Technique will be of great value to pathologists, pathology residents in training and also experimental pathology researchers that rely upon this methodology to perform tissue analysis in research Bibliography Includes bibliographical references and index Notes English Print version record In Springer eBooks Subject Pathology, Surgical -- Technique Pathology, Surgical -- Laboratory manuals Frozen tissue sections. Frozen Sections -- methods Pathology, Surgical -- methods Frozen Sections MEDICAL -- Surgery -- General. Médecine. Frozen tissue sections Pathology, Surgical Pathology, Surgical -- Technique Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Peters, Stephen R. ISBN 9781441912343 1441912347 1282827987 9781282827981 9786612827983 661282798X

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