Title Protein phosphatase protocols / edited by John W. Ludlow

Published Totowa, N.J. : Humana Press, ©1998

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Description 1 online resource (xiii, 316 pages) : illustrations Series Methods in molecular biology ; v. 93 Methods in molecular biology (Clifton, N.J.) ; v. 93 Contents Prokaryotic protein-serine/threonine phosphatases / Peter J. Kennelly -- Protein phosphatase type 1 and Type 2A assays / S. Derek Killilea, Qi Cheng, Zhi-Xin Wang -- Analyzing gene expression with the use of serine/threonine phosphatase inhibitors / Axel H. Schönthal -- Inhibitor-1, a regulator of protein phosphatase 1 function / John H. Connor, Hai Quan, Carey Oliver, Shirish Shenolikar -- I₁ PP2A and I₂ PP2A : two potent protein phosphatase 2A-specific inhibitor proteins / Mei Li, Zahi Damuni -- Control of PP1 activity through phosphorylation by cyclin-dependent kinases / Norbert Berndt -- Regulation of neuronal PP1 and PP2A during development / Elizabeth Collins, Alistair T. Sim -- PTPA regulating PP2A as a dual specificity phosphatase / Veerle Janssens, Christine Hoof, Wilfried Merlevede, Jozef Goris -- Microinjection and immunological methods in the analysis of type 1 and 2A protein phosphatases from mammalian cells / Patric Turowski, Ned J. Lamb -- Use of immunocomplexed substrate for detecting PP1 activity / John W. Ludlow, Deirdre A. Nelson, Nancy A. Krucher -- The biochemical identification and characterization of new species of protein phosphatase 1 / Monique Beullens, Willy Stalmans, Mathieu Bollen -- The relationship between insulin signaling and protein phosphatase 1 activation / Louis Ragolia, Najma Begum -- Analysis of the isoforms of protein phosphatase 1 (PP1) with polyclonal peptide antibodies / Margherita Tognarini, Emma Villa-Moruzzi -- Expression of mouse protein phosphatase 2C in Eschericia coli and COS 7 cells / Takayasu Kobayashi, Kazuyuki Kusuda, Motoko Ohnishi, Naoki Chida, Shinri Tamura -- Expression of functional protein phosphatase 1 catalytic subunit in E. coli / Mariam Dohadwala, Norbert Berndt -- Protein phosphatase 2A and protein phosphatase X genes in Arabidopsis thaliana / Gemma Pujol, Albert Ferrer, Joaquin Ariño -- Separation of protein phosphatase Type 2C isozymes by chromatography on blue sepharose / Susanne Klumpp, Dagmar Selke -- Chromatographic isolation of PP2A from Limulus lateral eyes : conventional and small scale methods / Samuel C. Edwards, Travis B. Dyke, Timothy H. Dyke, David L. Brautigan -- Purification and assay of the Ptc1/Tpd1 protein phosphatase 2C from the yeast Saccharomyces cerevisiae / Matthew K. Robinson, Eric M. Phizicky -- Molecular cloning of protein phosphatase type 2C isoforms from retinal cDNA / Dagmar Selke, Susanne Klumpp, Benjamin Kaupp, Arnd Baumann -- Analysis of protein interactions between protein phosphatase 1 and noncatalytic subunits using the yeast two-hybrid assay / Nadja T. Ramaswamy, Brian K. Dalley, John F. Cannon -- Identifying protein phosphatase 2A interacting proteins using the yeast two-hybrid method / Brent McCright, David M. Virshup -- Protein phosphatase 2A regulatory subunits : cDNA cloning and analysis of mRNA expression / Julie A. Zaucha, Ryan S. Westphal, Brian E. Wadzinski -- Synthetic lethal screening in protein phosphatase pathways / Jianhong Zheng, John F. Cannon -- The search for the biological function of novel yeast Ser/Thr phosphatases / Joaquin Ariño, Francesc Posas, Josep Clotet Summary In Protein Phosphatase Protocols, John Ludlow assembles a collection of cutting-edge techniques for investigating the structure and function of protein serine/threonine phosphatases. These methods are designed to enable the investigator to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources. Written by leading researchers with extensive practice in their use, each method is time-tested and sufficiently detailed to ensure reproducibility for both new and established investigators. The book also includes a discussion of the functional significance of this group of enzymes with respect to cell growth and development. Protein Phosphatase Protocols offers researchers the single best collection of step-by-step laboratory protocols now available for these important enzymes. Because of their easy reproducibility, these methods will greatly facilitate research in the area, and thus are certain to become indispensable for all those working to unlock the structure and function of the protein serine/threonine phosphatases Analysis fosforylering phosphorylation eiwitten proteins serine threonine phosphoric monoester hydrolases biologische technieken biological techniques protocollen protocols Molecular Biology (General) Biochemistry Moleculaire biologie (algemeen) Biochemie Bibliography Includes bibliographical references and index Notes English Print version record Subject Phosphoprotein phosphatases -- Laboratory manuals Phosphoprotein Phosphatases -- physiology SCIENCE -- Life Sciences -- Biochemistry. Phosphoprotein phosphatases Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Ludlow, John W. ISBN 0585276935 9780585276939 9781592592678 1592592678 9780896034686 0896034682 1280836008 9781280836008 9786610836000 6610836000

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