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Title Photosynthesis : methods and protocols / edited by Sarah Covshoff
Published New York, N.Y. : Humana Press : Springer, [2018]


Description 1 online resource (xi, 355 pages) : illustrations (chiefly color)
Series Methods in molecular biology, 1940-6029 ; v. 1770
Methods in molecular biology (Clifton, N.J.) ; v. 1770.
Contents Survey of tools for measuring in vivo photosynthesis / Berkley J. Walker, Florian A. Busch, Steven M. Driever, Johannes Kromdijk, and Tracy Lawson -- Photosynthetic gas exchange in land plants at the leaf level / Florian A. Busch -- Design and use of a digitally controlled device for accurate, multiplexed gas exchange measurements of the complete foliar parts of plants / Gavin M. George, Katharina Ko·lling, Roland Kuenzli, Matthias Hirsch-Hoffmann, Patrick Flu·tsch, and Samuel C. Zeeman -- Measuring canopy gas exchange using CAnopy Photosynthesis and Transpiration Systems (CAPTS) / Qingfeng Song and Xin-Guang Zhu -- Light-response curves in land plants / Robert A. Coe and HsiangChun Lin -- Chlorophyll fluorescence on the fast timescale / Olubukola O. Ajigboye, Rumiana V. Ray, and Erik H. Murchie -- Sub-saturating multiphase flash irradiances to estimate maximum fluorescence yield / Thomas J. Avenson and Aaron J. Saathoff -- Chlorophyll fluorescence imaging / Tracy Lawson and Silvere Vialet-Chabrand -- Measurement of O2 uptake and evolution in leaves in vivo using stable isotopes and membrane inlet mass spectrometry / Steven M. Driever and Neil R. Baker -- Using stable carbon isotopes to study C3 and C4 photosynthesis : models and calculations / Nerea Ubierna, Meisha-Marika Holloway-Phillips, and Graham D. Farquhar -- Liquid-phase measurements of photosynthetic oxygen evolution / Dmitriy Shevela, Wolfgang P. Schro·der, and Johannes Messinger -- Quantification of photosynthetic enzymes in leaf extracts by immunoblotting / J. Alejandro Perdomo, Cristina R.G. Sales, and Elizabete Carmo-Silva -- Extraction of RuBisCO to determine catalytic constants / Douglas J. Orr and Elizabete Carmo-Silva -- Spectrophotometric determination of RuBisCO activity and activation state in leaf extracts / Cristina R.G. Sales, Gustaf E. Degen, Anabela Bernardes da Silva, and Elizabete Carmo-Silva -- Creating leaf cell suspensions for characterization of mesophyll and bundle sheath cellular features / Roxana Khoshravesh and Tammy L. Sage -- Determining the subcellular localization of fluorescently tagged proteins using protoplasts extracted from transiently transformed Nicotiana benthamiana leaves / Vivien Rolland -- 3D clearing and molecular labeling in plant tissues / William M. Palmer, Jamie R. Flynn, Antony P. Martin, Stephanie L. Reed, Christopher P.L. Grof, Rosemary G. White, and Robert T. Furbank -- Evaluation of lipids for the study of photosynthetic membranes / Helmut Kirchhoff and Robert Yarbrough -- "Click" methodology for the functionalization of water oxidation catalyst iIridium oxide nanoparticles with hydrophobic dyes for artificial photosynthetic constructs / Jackson D. Megiatto Jr. and Catia Ornelas -- Biophotovoltaics : design and study of bioelectrochemical systems for biotechnological applications and metabolic investigation / Stephen J.L. Rowden, Paolo Bombelli, and Christopher J. Howe
Summary This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol viewed July 9, 2018)
Subject Photosynthesis -- Laboratory manuals
Plant proteins.
Plant Proteins
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Plant proteins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Covshoff, Sarah, editor
LC no. 2018940274
ISBN 9781493977864