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Title Photosynthesis research protocols / edited by Robert Carpentier
Published Totowa, N.J. : Humana Press, ©2004


Description 1 online resource (xiii, 344 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; v. 274
Methods in molecular biology (Clifton, N.J.) ; v. 274
Contents Fractionation of thylakoid membranes into grana and stroma thylakoids / Juan Cuello and Maria Jose Quiles -- Isolation of photosystem I particles from spinach / Tetsuo Hiyama -- Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii / Velupillai M. Ramesh and Andrew N. Webber -- Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants / Yasusi Yamamoto, Shinsuke Sakuma, and Jian-Ren Shen -- Isolation of functional photosystem II core particles from the Cyanobacterium synechocystis sp. PCC 6803 / Dmitrii Vavilin -- Isolation of photosystem I reaction center preparation from spinach / Tetsuo Hiyama -- Isolation of photosystem II reaction center complexes from plants -- Michael Seibert, Inmaculada Yruela, and Rafael Picorel -- Isolation of photosystem I reaction center subunit polypeptide from spinach / Tetsuo Hiyama
Purification of the cytochrome b6f complex in oxygenic photosynthesis / Huamin Zhang and William A. Cramer -- Purification of plastocyanin and cytochrome c6 from plants, green algae, and cyanobacteria / Jose A. Navarro, Manuel Hervçs, and Miguel A. De la Rosa -- Preparation of native and recombinant light-harvesting chlorophyll-a/b complex / Wolfgang Rohle and Harald Paulsen -- Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macroassemblies with light-inducible structural transitions / Ilian Simidjiev, Zsuzsanna Vçrkonyi, and GyÜzÜ Garab -- Separation, purification and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) / Grzegorz Jackowski -- Isolation of CP43 and CP47 photosystem II proximal antenna complexes for plants / Rafael Picorel, Miguel Alfonso, and Michael Seibert
The determination and quantification of photosynthetic pigments by reverse phase high performance liquid chromatography (HPLC), thin layer chromatography, and spectroscopy / Tessa Pocock, Marianna Krùl, and Norman P.A. Huner -- Isolation and identification of chloroplast lipids / Sato and Mikio Tsuzuki -- DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents: synthesis, stability, and structural features / Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, and Stravoula Diamantoglou -- Incorporation and analysis of LHCII in model systems / Wieslaw I. Gruszecki -- Photosystem II reconstitution into proteoliposomes: structure-function characterization / Mario Fragata -- Extraction of the functional manganese and calcium cofactors from photosystem II / Joel Freeman, Garth Hendry, and Tom Wydrzynski -- Assay of photoinhibition of photosystem II and protease activity / Yasusi Yamamoto, Yoji Nishi, Hitoshi Yamasaki, Suguru Uchida, and Satoshi Ohira
Thermoluminescence: a technique for probing photosystem II / Prafullachandra V. Sane -- Detection of free radicals and reactive oxygen species / Eva Hideg -- Stabilization of photosynthetic materials / Regis Rouillon, Pierre Euzet, and Robert Carpentier -- Determination of phosphoproteins in higher plant thylakoids / Eva-Mari Aro, Anne Rokka, and Alexander V. Vener -- Identifying photoprotection mutants in Arabidopsis thaliana / Jan Bart Rossel, Abby Cuttriss, and Barry J. Pogson -- A simple method for chloroplast transformation in Chlamydomonas reinhardtii / Velupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber -- Construction of gene knockouts in the Cyanobacterium synechocystis pcc 6803 / Julian J. Eaton-Rye -- Gene inactivation in the cyanobacterium synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation / Niels-Ulrik Frigaard, Yumiko Sakuragi, and Donald A. Bryant
Summary Because photosynthesis is at the heart of plant bioenergetics, fundamental to plant productivity and biomass, and a major factor in global climate, it is widely studied across the many fields it embraces. In Photosynthesis Research Protocols, well-known researchers describe in step-by-step detail a broad range of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. The methods presented are fundamental for research work in agriculture, biochemistry, biotechnology, botany, cell biology, environmental science, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, and plant physiology. All protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Photosynthesis Research Protocols offers both newcomers and experienced researchers an up-to-date collection of readily reproducible techniques essential for photosynthesis research today
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Photosynthesis -- Laboratory manuals
Plant proteins.
Plant Proteins
SCIENCE -- Life Sciences -- Biochemistry.
Plant proteins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Carpentier, Robert, 1956-
ISBN 1588292320