| Description |
1 online resource (xi, 180 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1405 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 1405. 1064-3745
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Springer protocols (Series)
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| Contents |
Elicitation approaches for withanolide production in hairy root culture of Withania somnifera (L.) Dunal / Ganeshan Sivanandhan [and others] -- Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction / Thanise Nogueira Füller [and others] -- Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines / Júlio César de Lima [and others] -- Collection of apoplastic fluids from Arabidopsis thaliana leaves / Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier -- From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica / Fernanda de Costa [and others] -- Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / Flávia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera -- Heterologous expression and characterization of mimosinase from Leucaena leucocephala / Vishal Singh Negi and Dulal Borthakur -- Production of aromatic plant terpenoids in recombinant baker's yeast / Anita Emmerstorfer-Augustin and Harald Pichler -- Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography (IMAC) / Fernanda de Costa [and others] -- Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes / Morten Egevang Jørgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier -- Laser capture microdissection : avoiding bias in analysis by selecting just what matters / Márcia R. de Almeida and Martina V. Strömvik -- Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants / Inês Carqueijeiro [and others] -- Protoplast transformation as a plant-transferable transient expression system / Patrícia Duarte [and others] -- Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system / Basdeo Bhagwat [and others] -- Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs / Ming Chi [and others] |
| Summary |
This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Plant cells and tissues -- Laboratory manuals
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Plant Development
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Plants -- metabolism
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Botany & plant sciences.
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Science -- Life Sciences -- Botany.
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Plant cells and tissues
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Fett-Neto, Arthur Germano, 1967-
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| ISBN |
9781493933938 |
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1493933930 |
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1493933914 |
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9781493933914 |
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