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Title Polyamine protocols / edited by David M.L. Morgan
Published Totowa, N.J. : Humana Press, ©1998


Description 1 online resource (ix, 186 pages) : illustrations
Series Methods in molecular biology ; v. 79
Methods in molecular biology (Clifton, N.J.) ; v. 79.
Contents Polyamines : an introduction / David M. Morgan -- Determination of ornithine decarboxylase activity using [³H]ornithine / Amalia Tabib -- Assay of mammalian ornithine decarboxylase activity using [¹⁴C]ornithine / Catherine S. Coleman, Anthony E. Pegg -- Assay of mammalian S-adenosylmethionine decarboxylase activity / Lisa M. Shantz, Anthony E. Pegg -- Assay of spermidine and spermine synthases / Laurie Wiest, Anthony E. Pegg -- Measurement of spermidine/spermine N¹-acetyltransferase activity / Heather M. Wallace, Deborah M. Evans -- Assay of spermidine N⁸-acetyltransferase / Jim Blankenship -- Assay of N⁸-acetylspermidine deacetylase / Jim Blankenship -- Hydrogen peroxide assay for amine oxidase activity / R. James Storer, Antonio Ferrante -- Radiochemical assay of diamine oxidase / R. James Storer, Antonio Ferrante -- Radiochemical estimation of polyamine oxidase / David M. Morgan -- Measurement of aldehyde formation as an assay for polyamine oxidase activity / David M. Morgan -- Determination of polyamines as their benzoylated derivatives by HPLC / David M. Morgan -- A dansyl chloride-HPLC method for the determination of polyamines / Karl J. Hunter -- The determination of polyamines and amino acids by a fluorescamine-HPLC method / Karl J. Hunter, Alan H. Fairlamb -- Thin-layer chromatographic method for assaying polyamides / Rentala Madhubala -- Measurement of polyamine transport. Adherent cells / David M. Morgan -- Measurement of polyamine transport. Cells in suspension / Sarah A. Quesne, Alan H. Fairlamb -- Measurement of polyamine efflux from cells in culture / Heather M. Wallace, A. Jill Mackarel -- Leucine incorporation and thymidine incorporation / Christopher P. Denton -- Tetrazolium (MTT) assay for cellular viability and activity / David M. Morgan
Summary David Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results. Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances
Analysis polyaminen
enzyme activity
Proteins and Enzymes
Eiwitten en enzymen
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
In Springer Protocols
Subject Polyamines -- Laboratory manuals
Polyamines -- analysis
Polyamines -- metabolism
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Morgan, David M. L
ISBN 9781592595655