| Description |
1 online resource (xiv, 523 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 720 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 720
|
| Contents |
Current status of the polyamine research field / Anthony E. Pegg and Robert A. Casere, Jr. -- Exploring polyamine biosynthetic diversity through comparative and functional genomics / Anthony J. Michael -- Characterization of genes for polyamine modulon / Kazuei Igarashi and Keiko Kashiwagi -- Posttranscriptional regulation of gene expression in epithelial cells by polyamines / Lan Xiao and Jian-Ying Wang -- Identification, chemical synthesis, and biological functions of unusual polyamines produced by extreme thermophiles / Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui -- Polyamine block of inwardly rectifying potassium channels / Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols -- Carcinogenesis studies in mice with genetically engineered alterations in polyamine metabolism / David J. Feith -- Transgenic rodents with altered SSAT expression as models of pancreatitis and altered glucose with lipid metabolism / Anne Uimari [and others] -- Use of (gyro) gy and spermine synthase transgenic mice to study functions of spermine / Xiaojing Wang and Anthony E. Pegg -- Simple assay for mammalian spermine oxidase : a polyamine catabolic enzyme implicated in drug response and disease / Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr. -- Characterization, assay, and substrate specificity of plant polyamine oxidases / Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis -- Assay of deoxyhypusine synthase activity / Jong Hwan Park, Edith C. Wolff, and Myung Hee Park -- Identification and assay of allosteric regulators of S-adenosylmethionine decarboxylase / Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips -- Protocols for studying antizyme expression and function / Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji -- Identification, assay and functional analysis of the antizyme inhibitor family / Chaim Kahana -- Posttranscriptional regulation of ornithine decarboxylase / Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz -- Identification and assays of polyamine transport systems in Escherichia coli and Saccharomyces cerevisiae / Keiko Kashiwagi and Kazuei Igarashi -- Genetic and biochemical analysis of protozoal polyamine transporters / Marie-Pierre Hasne and Buddy Ullman -- Heparan sulfate proteoglycan-mediated polyamine uptake / Johanna Welch [and others] -- Polyamine transport sysems in mammalian cells and tissues / Takeshi Uemura and Eugene W. Gerner -- Procedures to evaluate the importance of dietary polyamines / Paul Acheampong, Mary J. Macleod, and Heather M. Wallace -- Determination of N¹, N¹²-diacetylspermine in urine : a novel tumor marker / Masao Kawakita [and others] -- Spermidine/spermine-N¹-acetyltransferase in kidney ischemia reperfusion injury / Kamyar Zahedi and Manoocher Soleimani -- Use of polyamine metabolites as markers for stroke and renal failure / Kazuei Igarashi and Keiko Kashiwagi -- Methods to evaluate alterations in polyamine metabolism caused by Helicobacter pylori infection / Alain P. Gobert, Rupesh Chaturvedi, and Keith T. Wilson -- High-resolution capillary gas chromatography in combination with mass spectrometry for quantification of three major polyamines in postmortem brain cortex / Gary Gang Chen [and others] -- Spermine synthase deficiency resulting in X-linked intellectual disabiltiy (Snyder-Robinson Syndrome) / Charles E. Schwartz, Xaiojing Wang, Roger E. Stevenson, and Anthony E. Pegg -- Methylated polyamines as research tools / Alex R. Khomutov [and others] -- Fluorescent substrates for polyamine catabolic enzymes and transport / Koichi Takao and Akira Shirahata -- Use of polyamine derivatives as selective histone deacetylase inhibitors / Patick M. Woster -- Measurement of polyamine pKa values / Ian S. Blagbrough, Abdelkader A. Metwally, and Andrew J. Geall -- Polyamine analysis by LC-MS / Merja R. Hakkinen |
| Summary |
[Publisher-supplied data] Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development. In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study. The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology |
| Analysis |
polyaminen |
|
polyamines |
|
celbiologie |
|
cellular biology |
|
geneeskunde |
|
medicine |
|
Molecular Biology (General) |
|
Moleculaire biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Polyamines.
|
|
Spermine.
|
|
Polyamines
|
|
Spermine
|
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Spermine
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Polyamines
|
| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Pegg, Anthony E.
|
|
Casero, Robert Anthony.
|
| ISBN |
9781617790348 |
|
1617790346 |
|
9781617790331 |
|
1617790338 |
|
