Title Polyadenylation in plants : methods and protocols / edited by Arthur G. Hunt, Qingshun Quinn Li

Published New York : Humana Press : Springer, [2015] ©2015

Click on the following:
Springer eBooks

Copies

Description 1 online resource (ix, 225 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 1255 Methods in molecular biology (Clifton, N.J.) ; v. 1255. 1064-3745 Contents Computational analysis of plant polyadenylation signals / Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li -- Prediction of plant mRNA polyadenylation sites / Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li -- Extraction of poly(A) sites from large-scale RNA-seq data / Min Dong [and others] -- Poly(A)-tag deep sequencing data processing to extract poly(A) sites / Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li -- Analysis of poly(A) site choice using a java-based clustering algorithm / Patrick E. Thomas -- RADPRE : a computational program for identification of differential mRNA processing including alternative polyadenylation / Denghui Xing and Qingshun Quinn Li -- Characterization of plant polyadenylation complexes by using tandem affinity purification / Hongwei Zhao, Xinfu Ye, and Qingshun Quinn Li -- In vitro analysis of cleavage and polyadenylation in Arabidopsis / Hongwei Zhao and Qingshun Quinn Li -- Production, purification, and assay of recombinant proteins for in vitro biochemical analyses of the plant polyadenylation complex / Stephen A. Bell and Balasubrahmanyam Addepalli -- Detection of disulfide linkage by chemical derivatization and mass spectrometry / Balasubrahmanyam Addepalli -- Transient expression using agroinfiltration to study polyadenylation in plants / Carol Von Lanken and Arthur G. Hunt -- 3' RACE protocol to confirm polyadenylation sites / Liuyin Ma and Arthur G. Hunt -- Phage display library screening for identification of interacting protein partners / Balasubrahmanyam Addepalli, Suryadevara Rao, and Arthur G. Hunt -- Genome- wide determination of poly(A) site choice in plants / Pratap Kumar Pati, Liuyin Ma, and Arthur G. Hunt -- DNA/RNA hybrid primer mediated poly(A) tag library construction for illumina sequencing / Man Liu, Xiaohui Wu, and Qingshun Quinn Li -- Poly(A) tag library construction from 10 ng total RNA / Jingyi Cao and Qingshun Quinn Li -- Rapid, simple, and inexpensive method for the preparation of strand-specific RNA-seq libraries / Arthur G. Hunt -- Genome-wide analysis of distribution of RNA polymerase II isoforms using ChIP-Seq / Laura de Lorenzo Summary This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. Numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Some are specific for plant research, but most can be adopted for research in other organisms. Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3'-end formation in plants Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed January 5, 2014) Subject Plant gene expression -- Laboratory manuals Plants Polyadenylation vegetation. Plant gene expression Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hunt, Arthur G., editor Li, Qingshun Quinn, editor LC no. 2014956397 ISBN 9781493921751 1493921754 1493921746 9781493921744

  Permalink