Title Forensic DNA typing protocols / edited by Angel Carracedo

Published Totowa, N.J. : Humana Press, ©2005

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Description 1 online resource (x, 280 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 297 Methods in molecular biology (Clifton, N.J.) ; v. 297 Contents Forensic DNA-typing technologies: a review -- DNA extraction and quantitation of forensic samples using the phenol-chloroform method and real-time PCR -- A real-time PCR protocol to determine the number of amelogenine (X-Y) gene copies from forensic DNA samples -- Species determination: the role and use of the cytochrome b gene -- Constructing STR mulitplex assays -- Y chromosome STR typing -- Using online databases for developing SNP markers of forensic interest SNP typing in forensic genetics: a review -- Genotyping SNPs with the lightcycler -- Universal tag arrays in forensic SNP analysis -- SNP typing on the nanochip electronic microarray -- Genotyping SNPs using a UV-photocleavable oligonucleotide in MALDI-TOF MS -- Mitochondrial D-loop and coding sequence analysis using pyrosequencing -- SNaPshot typing of mitochondrial DNA coding region variants -- Typing of Y chromosome SNPs with multiplex PCR methods Y chromosome SNP analysis using single-base extension: a hierarchical multiplex design -- SNaPshot for pharmacogenetics by minisequencing -- Methods for the study of ancient DNA -- Protocols for ancient DNAtyping Summary In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science Bibliography Includes bibliographical references and index Notes English Print version record Subject DNA fingerprinting. Forensic genetics. DNA Fingerprinting -- methods Polymorphism, Single Nucleotide -- genetics DNA Fingerprinting MEDICAL -- Forensic Medicine. DNA fingerprinting Forensic genetics Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Carracedo, Ángel, 1955- ISBN 1588292649 9781588292643 1592598676 9781592598670 9786610359493 6610359490

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