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Title Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler
Published San Diego : Academic Press, ©1999

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Description 1 online resource (xii, 480 pages) : illustrations
Contents Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index
Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants
Summary Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
English
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Gene expression.
Prokaryotes -- Genetics
Insects -- Genetics
Transgenic animals -- Genetics
Eukaryotic cells.
Genetic regulation.
Prokaryotic Cells
Gene Expression Regulation
Gene Expression
Animals, Genetically Modified -- genetics
Eukaryotic Cells
SCIENCE -- Life Sciences -- Genetics & Genomics.
Eukaryotic cells
Gene expression
Insects -- Genetics
Prokaryotes -- Genetics
Genexpressie.
Genregulatie.
Expression génique.
Procaryotes -- Génétique.
Insectes.
Animaux transgéniques.
Cellules eucaryotes.
Form Electronic book
Author Fernandez, Joseph M.
Hoeffler, James P.
LC no. 98085621
ISBN 9780122538407
0122538404
9780080532356
0080532357
1281054186
9781281054180
9786611054182
6611054189