Limit search to available items
Book Cover
E-book

Title DNA repair protocols : prokaryotic systems / edited by Pat Vaughan
Published Totowa, N.J. : Humana Press, ©2000

Copies

Description 1 online resource (x, 209 pages) : illustrations
Series Methods in molecular biology ; 152
Methods in molecular biology (Clifton, N.J.) ; v. 152
Contents pt. 1. Assays to detect and quantitate DNA repair activity -- Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase -- Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1) -- Detection and quantitation of uracil DNA glycosylase activity -- Detection of DNA deoxyribophosphodiesterase activity -- O⁶-alkyguanine-DNA alkyltransferase assay -- The E. coli vsr endonuclease: assaying activity in vitro and in vivo -- Coupling of DNA helicase function to DNA strand exchange activity -- Detection and quanitation of recBCD enzymer (exonuclease V) activity -- Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima -- MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance -- pt. 2. Use of DNA repair enzymes as tools in molecular biology -- Flexible genetic engineering using RecA protein -- Interspecies recombination and mismatch repair: generation of mosaic genes and genomes -- The use of immobilized mismatch binding protein in mutation/SNP detection -- Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check) -- Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan) -- The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection
Summary DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology
Analysis dna repair
prokaryoten
prokaryotes
enzymen
enzymes
moleculaire genetica
molecular genetics
biologische technieken
biological techniques
protocollen
protocols
Nucleic Acids
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject DNA repair -- Laboratory manuals
DNA repair.
DNA Repair
Prokoryotic Cells -- cytology
SCIENCE -- Life Sciences -- Genetics & Genomics.
DNA repair
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Vaughan, Pat
ISBN 1592590683
9781592590681
0585354332
9780585354330
089603643X
9780896036437
1280820594
9781280820595
9786610820597
6610820597