Title DNA repair protocols : prokaryotic systems / edited by Pat Vaughan

Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (x, 209 pages) : illustrations Series Methods in molecular biology ; 152 Methods in molecular biology (Clifton, N.J.) ; v. 152 Contents pt. 1. Assays to detect and quantitate DNA repair activity -- Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase -- Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1) -- Detection and quantitation of uracil DNA glycosylase activity -- Detection of DNA deoxyribophosphodiesterase activity -- O⁶-alkyguanine-DNA alkyltransferase assay -- The E. coli vsr endonuclease: assaying activity in vitro and in vivo -- Coupling of DNA helicase function to DNA strand exchange activity -- Detection and quanitation of recBCD enzymer (exonuclease V) activity -- Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima -- MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance -- pt. 2. Use of DNA repair enzymes as tools in molecular biology -- Flexible genetic engineering using RecA protein -- Interspecies recombination and mismatch repair: generation of mosaic genes and genomes -- The use of immobilized mismatch binding protein in mutation/SNP detection -- Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check) -- Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan) -- The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection Summary DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology Analysis dna repair prokaryoten prokaryotes enzymen enzymes moleculaire genetica molecular genetics biologische technieken biological techniques protocollen protocols Nucleic Acids Bibliography Includes bibliographical references and index Notes English Print version record Subject DNA repair -- Laboratory manuals DNA repair. DNA Repair Prokoryotic Cells -- cytology SCIENCE -- Life Sciences -- Genetics & Genomics. DNA repair Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Vaughan, Pat ISBN 1592590683 9781592590681 0585354332 9780585354330 089603643X 9780896036437 1280820594 9781280820595 9786610820597 6610820597

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