Title MHC Volume 1 : a practical approach / edited by N. Fernandez and G. Butcher

Published Oxford ; New York : Oxford University Press, ©1997

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Description 1 online resource Series Practical approach series ; 180 Practical approach series ; 180. Contents Intro; Contents; Contributors; Abbreviations; 1. Isolation and functional properties of antigen presenting cells; 1. Introduction; 2. Isolation and functional properties of antigen presenting cells; Dendritic cells; Macrophages; B cells; 3. Functional assays of antigen presentation; Presentation requirements for activation of C5-specific T cell hybrids and clones; Readouts for T cell activation; References; 2. Labelling methods for analysis of class II MHC endocytosis and peptide turnover; 1. Introduction; 2. Surface labelling of MHC molecules Lactoperoxidase-catalysed labelling on tyrosine residuesLabelling of amino groups by succinimidyl ester based reagents; Methods utilized for study of the endocytosis of class I and II glycoproteins in B-lymphoblastoid cells; Study of the endocytosis of class I and II MHC glycoproteins in B-lymphoblastoid cells using cleavable cell surface labelling reagents; Endocytosis of the MHC glycoproteins in the presence of primaquine; Measurement of the recycling kinetics of endocytosed class I and II MHC glycoproteins; Study of the antigenic peptides associated with class II MHC glycoproteins Analysis of the turnover time of class II MHC and its associated peptidesAcknowledgements; References; 3. Methods for detecting signalling via MHC class II molecules which can lead to either activation or programmed cell death; 1. Introduction; 2. Changes in intracellular calcium levels in signal transduction; 3. Inositol phospholipid turnover during lymphocyte activation; 4. Signal transduction by kinases and phosphatases; 5. Detection of tyrosine kinase activity; 6. Immunoprecipitation of tyrosine-phosphorylated substrates; 7. Detection of programmed cell death via MHC class II molecules 8. A flow cytometric method for the quantification of cell death9. Visualization of programmed cell death by fluorescence microscopy; Summary; References; 4. Peptide translocation into the ER; 1. Introduction; 2. TAP-dependent peptide translocation; Peptide translocation in streptolysin O-permeabilized cells; Peptide translocation in microsomes; 3. Peptide substrates; Peptide size; The sequence of peptides; Peptide modifications; Competition for peptide translocation; References; 5. The 20S proteasome and antigen presentation; 1. Introduction Summary The major histocompatibility complex is the subject of much research in the immunology area. There is great interest in the structure of MHC proteins and in their function as antigen presenting molecules. Many immunology labs are now faced with the prospect of tackling biochemical and genetic techniques in order to study these molecules, and these two Practical Approach volumes provide the ideal introduction. MHC 1, Antigen Processing and Presentation, iscomplemented by MHC 2, Genes, Cells, and Molecules Bibliography Includes bibliographical references and indexes Subject Major histocompatibility complex -- Laboratory manuals Antigen presenting cells. Major histocompatibility complex. Antigen Presentation Major Histocompatibility Complex Antigen-Presenting Cells Proteins -- chemistry MEDICAL -- Immunology. Antigen presenting cells Major histocompatibility complex Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Fernandez, Nelson, Dr. Butcher, G. (Geoffrey W.) ISBN 0585483809 9780585483801 9780191565526 0191565520

Other Titles Title on series title page: MHC 1

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