Title Capillary electrophoresis of proteins and peptides / edited by Mark A. Strege, Avinash L. Lagu

Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xi, 332 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; v. 276 Methods in molecular biology (Clifton, N.J.) ; v. 276 Contents Surfactant based methods for prevention of protein adsorption in capillary electrophoresis / Charles A. Lucy, Nicole E. Baryla, and Ken K.-C. Yeung -- Capillary coating for protein separation based on Si-O and Si-C covalent bond formation for capillary electrophoresis with laser-induced fluorescence detection / Hossein Ahmadzadeh, Norman J. Dovichi, and Sergey Krylov -- On-column labeling reaction for the analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection / Hossein Ahmadzadeh and Sergey Krylov Covalent and noncovalent labeling schemes for near infrared dyes in capillary electrophoresis protein applications / John Sowell [and others] -- Capillary electrophoresis in the analysis and monitoring of biotechnological processes / Vadim Klyushnichenko -- Capillary electrophoresis of proteins in a quality control environment / David L. Good [and others] -- Analysis of neutral N-linked oligosaccharides from antibodies using free-solution capillary electrophoresis in bare fused silica capillaries / Jeffrey S. Patrick [and others] Affinity capillary electrophoresis to examine receptor-ligand interactions / Maryam Azad [and others] -- Screening major binding sites on human serum albumin by affinity capillary electrophoresis / Hee Seung Kim, John Austin, and David S. Hage -- Using charge ladders and capillary electrophoresis to measure the charge, size, and electrostatic interactions of proteins / Upma Sharma and Jeffrey D. Carbeck -- Frontal analysis continuous capillary electrophoresis for protein-polyelectrolyte binding studies / Emek Seyrek, Toshiaki Hattori, and Paul L. Dubin -- Analysis of proteins by CE, CIEF, and microfluidic devices with whole column imaging detection / Jiaqi Wu [and others] Capillary electrophoresis electrospray ionization mass spectrometry of amino acids, peptides, and proteins / Mehdi Moini -- Capillary isoelectric focusing -- mass spectrometry of proteins and protein complexes / Suzana Martinovic, Ljiljana Pasa-Tolic, and Richard D. Smith -- Integrated system for rapid proteomics analyses using microfluidics devices coupled to nanoelectrospray mass spectrometry / Jianjun Li [and others] Summary Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Capillary electrophoresis -- Laboratory manuals Proteins -- Separation -- Laboratory manuals Peptides -- Separation -- Laboratory manuals Electrophoresis, Capillary -- methods Proteins -- analysis SCIENCE -- Life Sciences -- Biochemistry. Capillary electrophoresis Peptides -- Separation Proteins -- Separation Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Strege, Mark A Lagu, Avinash L ISBN 1588290174 9781588290175 159259798X 9781592597987 1280358254 9781280358258 9786610358250 6610358257

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