| Description |
1 online resource (xxvi, 441 pages) : illustrations (some color) |
| Contents |
Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting |
| Summary |
The fifth edition of this successful text has been revised and expanded, omitting some topics on obsolete DNA electrophoresis. It features a section on electrophoretic micro-methods and on-the-chip electrophoresis. It is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (John Wiley, viewed March 7, 2016) |
| Subject |
Electrophoresis -- Laboratory manuals
|
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DNA -- Separation -- Laboratory manuals
|
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Proteins -- Separation -- Laboratory manuals
|
|
Electrophoresis.
|
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Electrophoresis
|
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SCIENCE -- Chemistry -- Physical & Theoretical.
|
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DNA -- Separation
|
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Electrophoresis
|
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Proteins -- Separation
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| ISBN |
9783527695188 |
|
3527695184 |
|
9783527695164 |
|
3527695168 |
|
9783527695195 |
|
3527695192 |
|
9783527695171 |
|
3527695176 |
|
3527338802 |
|
9783527338801 |
|
