Title Purinergic signaling : methods and protocols / edited by Pablo Pelegrín

Published New York, NY : Humana Press, [2020]

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Description 1 online resource (xii, 360 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; 2041 Methods in molecular biology (Clifton, N.J.) ; v. 2041. 1064-3745 Summary This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed January 25, 2021) Subject Purines -- Receptors -- Laboratory manuals Purine nucleotides -- Laboratory manuals Purine nucleosides -- Laboratory manuals Receptors, Purinergic P2X Purinergic Agonists Purinergic Antagonists Purine nucleosides Purine nucleotides Purines -- Receptors Form Electronic book Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Author Pelegrín, Pablo, editor ISBN 9781493997176 1493997173

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