| Description |
1 online resource (xi, 344 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ; 725 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 725. 1064-3745
|
| Contents |
Purification of native argonaute complexes from the fission yeast Schizosaccharomyces pombe / Shane M. Buker and Mohammad R. Motamedi -- Chromatin immunoprecipitation in fission yeast / Thomas A. Volpe and Jessica DeMaio -- Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-argonaute monoclonal antibodies / Keita Miyoshi [and others] -- Mapping of Ago2-GW182 functional interactions / Bing Yao [and others] -- Continuous density gradients to study argonaute and GW182 complexes associated with the endocytic pathway / Derrick Gibbings -- In vitro RISC cleavage assay / Julia Stoehr and Gunter Meister -- Native gel analysis for RISC assembly / Tomoko Kawamata and Yukihide Tomari -- Purification and assembly of human argonaute, dicer, and TRBP complexes / Nabanita De and Ian J. MacRae -- Detection of human dicer and argonaute 2 catalytic activity / Marjorie P. Perron [and others] -- Imaging the cellular dynamics of drosophila argonaute proteins / Jing Li [and others] -- Live Cell imaging of argonaute proteins in mammalian cells / Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman -- Reporter-based assays for analyzing RNA interference in mammalian cells / Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan -- Artificial tethering of argonaute proteins for studying their role in translational repression of target mRNAs / Stephanie Eckhardt [and others] -- Efficient system for Let-7 microRNA and GW182 protein-mediated deadenylation in vitro / Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg -- Cell-free microRNA-mediated translation repression in Caenorhabditis elegans / Edlyn Wu and Thomas F. Duchaine -- Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices / Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard -- Cloning argonaute-associated small RNAs from Caenorhabditis elegans / Weifeng Gu [and others] -- Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs / Yohei Kirino [and others] -- Generation of an inducible mouse ES cell lines deficient for argonaute proteins / Hong Su and Xiaozhong Wang -- Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach / Olivia A. Ebner and Matthias Selbach |
| Summary |
With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology"!series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research |
| Analysis |
eiwitten |
|
proteins |
|
genetische modificatie |
|
genetic engineering |
|
protocollen |
|
protocols |
|
Proteins and Enzymes |
|
Eiwitten en enzymen |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Proteins -- Analysis.
|
|
RNA interference.
|
|
Argonaute Proteins
|
|
RNA Interference
|
|
RNA-Binding Proteins
|
|
Proteins -- Analysis
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hobman, Tom C., 1961-
|
|
Duchaine, Thomas, 1974-
|
| ISBN |
9781617790461 |
|
161779046X |
|
1617790451 |
|
9781617790454 |
|
