Title MicroRNA protocols / edited by Shao-Yao Ying

Edition 2nd ed Published New York : Humana Press : Springer, ©2013

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Description 1 online resource (xi, 375 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; v. 936 Methods in molecular biology (Clifton, N.J.) ; volume 936. 1064-3745 Contents MicroRNA / Shao-Yao Ying, Donald C. Chang, and Shi-Lung Lin -- Analysis of MicroRNA length variety generated by recombinant human dicer / Julia Starega-Roslan and Wlodzimierz J. Krzyzosiak -- User-friendly computational workflow for the analysis of MicroRNA deep sequencing data / Anna Majer, Kyle A. Caligiuri, and Stephanie A. Booth -- RNA fene information : retroelement-MicroRNA entangling as the RNA quantum code / Yoichi Robertus Fujii -- Setting up an intronic miRNA database / Ludwig Christian Hinske [and others] -- Identify intronic MicroRNA with bioinformatics / Chih-Hao Kuo [and others] -- Experimental miRNA target validation / Jens Heyn [and others] -- Enrichment analysis of miRNA targets / Jianzhen Xu and Chi-Wai Wong -- MicroRNA expression profiling during neural differentiation of mouse embryonic carcinoma P19 cells / Botao Zhao, Bing Huang, Wei Li, and Youxin Jin -- Evaluating the MicroRNA targeting sites by luciferase reporter gene assay / Yi Jin, Zujian Chen, Xiqiang Liu, and Xiaofeng Zhou -- Expression pattern analysis of MicroRNAs in Caenorhabditis elegans / Meltem Isik and Eugene Berezikov -- Use of viral systems to study miRNA-mediated regulation of gene expression in human cells / Eleonora Forte and Micah A. Luftig -- Cloning, expression, and functional analysis of genomic miRNA using retroviral system in cancer cells / Abdol Hossein Rezaeian, Yuan Gao, and Hui-Kuan Lin -- Preparing synaptoneurosomes from adult mouse forebrain / Giovanni Lugli and Neil R. Smalheiser -- Isolation of total RNA and detection procedures for miRNA present in bovine-cultured adipocytes and adipose tissues / Scott L. Pratt [and others] -- miRNA and shRNA expression vectors based on mRNA and miRNA processing / Ping Wu [and others] -- Gene silencing in vitro and in vivo using intronic MicroRNAs / Shi-Lung Lin and Shao-Yao Ying -- Vector-free methods for manipulating miRNA activity in vitro and in vivo / Angeliki Pantazi and Athanasios Zovoilis -- MicroRNA expression profiling of human-induced pluripotent and embryonic stem cells / Amit Sharma and Joseph C. Wu -- MicroRNA expression during neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonic carcinoma P19 cells / Hirohiko Hohjoh -- Isolation and identification of gene-specific MicroRNAs / Shi-Lung Lin, Donald C. Chang, and Shao-Yao Ying -- Transgene-like animal models using intronic MicroRNAs / Shi-Lung Lin, Shin-Ju E. Chang, and Shao-Yao Ying -- Mechanism and method for generating tumor-free iPS cells using intronic MicroRNA miR-302 Induction / Shi-Lung Lin and Shao-Yao Ying -- Salivary MicroRNAs and oral cancer detection / Janice M. Yoshizawa and David T.W. Wong -- Application of intronic MicroRNA agents in cosmetics / Jack S.K. Chen and David T.S. Wu -- MicroRNAs in skin and wound healing / Jaideep Banerjee and Chandan K. Sen -- MiRNA targets of prostate cancer / Jia Han Deng [and others] Summary Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain. In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study. The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls. Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields Analysis biomedische wetenschappen biomedicine genexpressie gene expression humane genetica human genetics Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Subject Small interfering RNA. Antisense RNA. Non-coding RNA. RNA. Nucleic acids. MicroRNA. Publication Formats RNA, Small Untranslated RNA, Antisense RNA, Untranslated Publication Characteristics RNA Antisense Elements (Genetics) Nucleic Acids, Nucleotides, and Nucleosides Nucleic Acids Chemicals and Drugs MicroRNAs Laboratory Manuals RNA, Small Interfering RNA Nucleic acids Non-coding RNA MicroRNA Antisense RNA Small interfering RNA Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Ying, Shao-Yao. ISBN 9781627030830 1627030832

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