| Description |
1 online resource (xi, 274 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1838 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1838. 1064-3745
|
| Contents |
Flow cytometry and direct sequencing of viruses / Mária Džunková -- Tissue-based universal virus detection (TUViD-VM) protocol for viral metagenomics / Claudia Kohl and Andreas Kurth -- Protocol for generating infectious RNA viromes from complex biological samples / Sonia Monteil-Bouchard, Sarah Temmam, and Christelle Desnues -- Phage on Tap : a quick and efficient protocol for the preparation of bacteriophage laboratory stocks / Natasha Bonilla and Jeremy J. Barr -- Extraction and purification of viruses from fecal samples for metagenome and morphology analyses / Josué L. Castro-Mejía, Ling Deng, Finn K. Vogensen, Alejandro Reyes, and Dennis S. Nielsen -- Virome sequencing of stool samples / Lenka Kramná and Ondřej Cinek -- NetoVIR : modular approach to customize sample preparation procedures for viral metagenomics / Nádia Conceição-Neto, Kwe Claude Yinda, Marc Van Ranst, and Jelle Matthijnssens -- Viral genome isolation from human faeces for succession assessment of the human gut virome / David Mayo-Muñoz -- Introduction to techniques and methodologies for characterizing the human respiratory virome / Alicia B. Mitchell and Allan R. Glanville -- Targeted sequencing of respiratory viruses in clinical specimens for pathogen identification and genome-wide analysis / Yu Yang, Shannon D. Walls, Stephen M. Gross, Gary P. Schroth, Richard G. Jarman, and Jun Hang -- Methods for enrichment and sequencing of oral viral assemblages : saliva, oral mucosa, and dental plaque viromes / Marcos Parras-Moltó and Alberto López-Bueno -- Method for isolation of the virome from plasma samples / Pilar Domingo-Calap and Juan Vicente Bou -- Viral concentration and amplification from human serum samples prior to application of next-generation sequencing analysis / Xavier Fernandez-Cassi, Marta Rusiñol, and Sandra Martínez-Puchol -- Identification and quantification of DNA viral populations in human urine using next-generation sequencing approaches / Tasha M. Santiago-Rodriguez -- Diversity analysis in viral metagenomes / Jorge Francisco Vázquez-Castellanos -- Construction of a comprehensive database from theexisting viral sequences available from the international nucleotide sequence database collaboration / Rodrigo García-López -- Robust analysis of time series in virome metagenomics / Jose Manuel Martí -- Bioinformatics assembling and assessment of novel coxsackievirus B1 genome / Jake Lin, Bryn Y. Kimura, Sami Oikarinen, and Matti Nykter |
| Summary |
This book details the most comprehensive, up-to -date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body. Chapters guide readers through methods on collection, isolation, identification and computational/statistical analysis, and body niches to cover those methodological issues inherent to the human tissues and organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Human Virome: Methods and Protocols aims to facilitate researchers with their daily work in the field of the research on the human virome |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 20,2018) |
| Subject |
Viral genetics -- Laboratory manuals
|
|
Viruses -- genetics
|
|
RNA, Viral
|
|
Metagenomics -- methods
|
|
Immunology.
|
|
Medical -- Immunology.
|
|
Viral genetics
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Moya, Andrés, editor
|
|
Brocal, Vicente Pérez, editor
|
| ISBN |
9781493986828 |
|
1493986821 |
|
