Description |
1 online resource (1 PDF file (x, 286 pages)) : illustrations |
Series |
Methods in molecular biology, 1940-6029 ; volume 2162 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 2162. 1064-3745
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Springer protocols (Series) 1949-2448
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Summary |
This volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls |
Bibliography |
Includes bibliographical references and index |
Notes |
Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL |
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Online resource; title from PDF title page (SpringerLink, viewed November 11, 2020) |
Subject |
RNA editing -- Laboratory manuals
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CRISPR (Genetics) -- Laboratory manuals
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Clustered Regularly Interspaced Short Palindromic Repeats
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RNA Editing
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Biomedical engineering.
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Genetics (non-medical).
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Technology & Engineering -- Biomedical.
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Science -- Life Sciences -- Genetics & Genomics.
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CRISPR (Genetics)
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RNA editing.
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Genre/Form |
Laboratory manuals.
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Fulga, Tudor A., editor
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Knapp, David J. H. F., editor
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Ferry, Quentin R. V., editor
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ISBN |
9781071606872 |
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1071606875 |
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