| Description |
1 online resource (1 PDF file (xi, 304 pages)) : illustrations |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; volume 2167 |
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Methods in molecular biology (Clifton, N.J.) ; v. 2167. 1064-3745
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Springer protocols (Series) 1949-2448
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| Contents |
Introduction -- Characterization of hammerhead ribozyme reactions -- Mechanistic analysis of the hepatitis delta virus (HDV) ribozyme: Methods for RNA preparation, structure mapping, solvent isotope effects, and co-transcriptional cleavage -- Kinetic characterization of hairpin ribozyme variants -- Characterization of RNase p rna activity -- Group I intron ribozymes -- Kinetic characterization of group II intron folding and splicing -- Mechanism and distribution of glmS ribozymes -- Structure-based search and in vitro analysis of self-cleaving ribozymes -- Discovery of rna motifs using a computational pipeline that allows insertions in paired regions and filtering of candidate sequences -- Crystallographic analysis of small ribozymes and riboswitches -- Functional dynamics of RNA ribozymes studied by NMR spectroscopy -- Deoxyribozyme-based, semisynthetic access to stable peptidyl-trnas exemplified by tRNA(Val) carrying a macrolide antibiotic resistance peptide -- Probing functionhe ribosomal peptidyl transferase center by nucleotide analog interference -- Single molecule fret characterization of large ribozyme folding -- Metal ion-RNA interactions studied via multinuclear NMR -- Analysis of catalytic RNA structure and function by nucleotide analog interference mapping -- In vitro selection of metal ion-selective dnazymes -- Selecting allosteric ribozymes -- Screening effective target sites on mRNA: A ribozyme library approach -- A computational approach to predict suitable target sites for trans-acting minimal hammerhead ribozymes -- Targeting mRNAs by engineered sequence-specific RNase p ribozymes -- Target-induced SOFA-HDV ribozyme -- Ribozyme-mediated trans insertion-splicing into target RNAs -- Developing fluorogenic RNA-cleaving dnazymes for biosensing applications -- Development of trainable deoxyribozyme-based game playing automaton -- Rational design and tuning of ribozyme-based devices -- In vivo screening of ligand-dependent hammerhead ribozymes -- Flexizymes as a tRNA acylation tool facilitating genetic code reprogramming |
| Summary |
This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide RNAs, AgoshRNAs and aptamers or to study RNA capping and long non-coding RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL |
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Description based on online resource; title from PDF title page (viewed August 25, 2021) |
| Subject |
Medicine.
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Human genetics.
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Genetic regulation.
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RNA, Catalytic
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Gene Expression Regulation
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Kinetics
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RNA Folding
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Medicine
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medicines (material)
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medicine (discipline)
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Medical genetics.
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Medical -- Genetics.
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Genetic regulation
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Human genetics
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Medicine
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Scarborough, Robert J., editor
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Gatignol, Anne, editor
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| ISBN |
9781071607169 |
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1071607162 |
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