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Title RNA interference, editing, and modification : methods and protocols / edited by Jonatha M. Gott
Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xiii, 435 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; 265
Methods in molecular biology (Clifton, N.J.) ; v. 265
Contents RNA interference: Historical overview and significance / M.K. Montgomery -- Methods for delivery of double-stranded rna into caenorhabditis elegans / D. Hull and L. Timmons -- Induction and biochemical purification of rna-induced silencing complex from drosophila s2 cells / A.A. Caudy and G.J. Hannon -- Analysis of gene function in trypanosoma brucei using rna interference / A. Djikeng [and others] -- Short hairpin activated gene silencing in mammalian cells / P.J. Paddison [and others] -- Geminivirus vectors for transient gene silencing in plants / N. Muangsan and D. Robertson -- Posttranscriptional gene silencing in plants / S.V. Wesley [and others] -- Identification of micrornas and other tiny noncoding rnas by cdna cloning / V. Ambros and R.C. Lee -- A historical perspective on rna editing: How the peculiar and bizarre became mainstream / D.J. Koslowsky -- Identification of substrates for adenosine deaminases that act on rna / D.P. Morse -- Purification and assay of recombinant adar proteins expressed in the yeast pichia pastoris or in escherichia coli / G.M. Ring, M.A. O'Connell and L.P. Keegan -- Isolation of an mrna-binding protein involved in c-to-u editing / C.A. Gerber, A. Relich and D.M. Driscoll -- In vitro assays for kinetoplastid u insertion-deletion editing and associated activities / K. Stuart [and others] -- Identification and characterization of trypanosome rna-editing complex components / K. Stuart, A.K. Panigrahi and A. Schnaufer -- Chimeric templates and assays used to study physarum cotranscriptional insertional editing in vitro / E.M. Byrne -- Methods for analysis of mitochondrial trna editing in acanthamoeba castellanii / A.J. Lohan and M.W. Gray -- In vitro rna editing systems from higher plant chloroplasts / T. Hirose [and others] -- Studying rna editing in transgenic chloroplasts of higher plants / R. Bock -- Detection and quantification of modified nucleotides in rna using thin-layer chromatography / H. Grosjean, G. Keith and L. Droogmans -- Functional characterization of 2'-o-methylation and pseudouridylation guide rnas / T. Kiss and B.E. Jady -- Experimental rnomics: A global approach to identifying small nuclear rnas and their targets in different model organisms / A. Huttenhofer, J. Cavaille and J.P. Bachellerie
Summary The study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed to study these processes in most of the major biological systems in which they are known to occur-a wide range of organisms that includes worms, flies, trypanosomes, mammals, and plants. A historical overview of each subject is provided to put related chapters into a larger context. RNA interference (RNAi) has also emerged in recent years as an important and promising tool for gene discovery and reverse genetic engineering. Application of this cutting-edge technique is described for a range of experimental systems. Topics of interest include stable and transient RNA interference, RNA editing, gene silencing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs, many of which are involved in RNA interference or modification. In addition, techniques used to study RNA editing mechanisms are elaborated for both systems that involve the conversion of one base to another and insertion-deletion editing. To promote the adaptation of their assays and approaches for use in other biological systems, the authors have included a range of methods representative of the major experimental systems in a given field. All protocols presented follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, RNA Interference, Editing, and Modification: Methods and Protocols offers not only geneticists, molecular biologists, and bioinformaticists a comprehensive collection of readily reproducible techniques for the elucidation of gene function and the alteration of gene expression, but also describes how best they may be productively used to address outstanding mechanistic questions and adapted to novel biological systems
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject RNA editing -- Laboratory manuals
RNA interference.
RNA interference -- Laboratory manuals
RNA Editing
RNA Interference
SCIENCE -- Life Sciences -- Molecular Biology.
RNA interference
RNA editing
Biologia molecular (métodos)
Manuais de laboratório.
Rna (genética)
Expressão gênica.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Gott, Jonatha M.
ISBN 9781592597758
1588292428
9781588292421
1592597750
1280360267
9781280360268
9786610360260
661036026X