| Description |
1 online resource (xii, 395 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1737 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1737. 1064-3745
|
| Contents |
Workflow for a computational analysis of an sRNA candidate in bacteria / Patrick R. Wright and Jens Georg -- Guidelines for inferring and characterizing a family of bacterial trans-acting small noncoding RNAs / Antonio Lagares Jr. and Claudio Valverde -- Bioinformatic approach for prediction of CsrA/RsmA-regulating small RNAs in bacteria / Carl T. Fakhry, Kourosh Zarringhalam, and Rahul V. Kulkarni -- Host-pathogen transcriptomics by dual RNA-Seq / Alexander J. Westermann and Jörg Vogel -- Identification of new bacterial small RNA targets using MS2 affinity purification coupled to RNA sequencing / Marie-Claude Carrier, Guillaume Laliberté, and Eric Massé -- Assessment of external guide sequences' (EGS) efficiency as inducers of RNase P-mediated cleavage of mRNA target molecules / Saumya Jani, Alexis Jackson, Carol Davies-Sala, Kevin Chiem, Alfonso Soler-Bistué, Angeles Zorreguieta, and Marcelo E. Tolmasky -- Evaluating the effect of small RNAs and associated chaperones on rho-dependent termination of transcription in vitro / Cédric Nadiras, Annie Schwartz, Mildred Delaleau, and Marc Boudvillain -- Mapping changes in cell surface protein expression through selective labeling of live cells / Pierre Fechter -- Fluorescence-based methods for characterizing RNA interactions in vivo / Abigail N. Leistra, Mia K. Mihailovic, and Lydia M. Contreras -- Mutational analysis of sRNA-mRNA base pairing by electrophoretic mobility shift assay / Eva Maria Sternkopf Lillebæk and Birgitte Haahr Kallipolitis -- Integrated cell-free assay to study translation regulation by small bacterial noncoding RNAs / Erich Michel, Olivier Duss, and Frédéric H.-T. Allain -- Quantitative super-resolution imaging of small RNAs in Bacterial Cells / Seongjin Park, Magda Bujnowska, Eric L. McLean, and Jingyi Fei -- Extraction and analysis of RNA isolated from pure bacteria-derived outer membrane vesicles / Janine Habier, Patrick May, Anna Heintz-Buschart, Anubrata Ghosal, Anke K. Wienecke-Baldacchino, Esther N.M. Nolte-'t Hoen, Paul Wilmes, and Joëlle V. Fritz -- Absolute regulatory small noncoding RNA concentration and decay rates measurements in Escherichia coli / Florent Busi, Véronique Arluison, and Philippe Régnier -- High-resolution, high-throughput analysis of Hfq-binding sites using UV crosslinking and analysis of cDNA (CRAC) / Brandon Sy, Julia Wong, Sander Granneman, David Tollervey, David Gally, and Jai J. Tree -- Producing Hfq/Sm proteins and sRNAs for structural and biophysical studies of ribonucleoprotein assembly / Kimberly A. Stanek and Cameron Mura -- Single-molecule FRET assay to observe the activity of proteins involved in RNA/RNA annealing / Thierry Bizebard, Véronique Arluison, and Ulrich Bockelmann -- Techniques to analyze sRNA protein cofactor self-assembly in vitro / David Partouche, Antoine Malabirade, Thomas Bizien, Marisela Velez, Sylvain Trépout, Sergio Marco, Valeria Militello, Christophe Sandt, Frank Wien, and Véronique Arluison -- Sequence-specific affinity chromatography of bacterial small regulatory RNA-binding proteins from bacterial cells / Jonathan Gans, Jonathan Osborne, Juliet Cheng, Louise Djapgne, and Amanda G. Oglesby-Sherrouse -- Identification of small RNA-protein partners in plant symbiotic bacteria / Marta Robledo, Ana M. Matia-González, Natalia I. García-Tomsig, and José I. Jiménez-Zurdo -- Modular genetic system for high-throughput profiling and engineering of multi-target small RNAs / Samuel D. Stimple, Ashwin Lahiry, Joseph E. Taris, David W. Wood, and Richard A. Lease |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed February 27, 2018) |
| Subject |
RNA -- Laboratory manuals
|
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RNA.
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RNA
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RNA, Bacterial
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Medical genetics.
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Medical -- Genetics.
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RNA
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Arluison, Veronique, editor
|
|
Valverde, Claudio, editor
|
| ISBN |
9781493976348 |
|
1493976346 |
|
9781493976331 |
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1493976338 |
|
