Description |
xiii, 264 pages ; 24 cm |
Series |
Methods in molecular biology ; v. 86 |
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Methods in molecular biology (Clifton, N.J.) ; v. 86
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Contents |
Machine derived contents note: Introduction to Isolating RNA -- Donald E. Macfarlane and Christopher E. Dahle. -- Large and Small Scale RNA Preparations from Eukaryotic Cells -- Wolfgang Uckert, Wolfgang Walther, and Ulrike Stein. -- An Improved Rapid Method of Isolating RNA from Cultured Cells -- David B. Batt, Gordon G. Carmichael, and Zhong Liu. -- Isolating RNA with the Cationic Surfactant, Catrimox-14 -- Christopher E. Dahle and Donald E. Macfarlane. -- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues -- Giorgio Stanta, Serena Bonin, and Rosella Perin. -- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides -- Shu-Hua Cheng and Jeffrey R. Seemann. -- Isolation of Plant Mitochondrial RNA from Green Leaves -- Fei Ye, Wolfgang O. Abel, and Ralf Reski. -- Extraction from Fresh and Frozen Blood -- Bimal D. M. Theophilus. -- Isolation of Total RNA from Bacteria -- John Heptinstall. -- Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel -- Tapas Mukhopadhyay and Jack A. Roth. -- Isolation of Messenger RNA -- Sian Bryant and David L. Manning -- Formaldehyde Gel Electrophoresis of Total RNA -- Sian Bryant and David L. Manning. -- UV Spectrophotometric Analysis of Ribonucleic Acids -- Ralph Rapley and John Heptinstall. -- Preparation of RNA Dot-Blots -- Rachel Hodge. -- Nonradioactive Northern Blotting -- Rainer Lw̲. -- The Use of RNA Probes for the Analysis of Gene Expression -- Dominique Belin. -- Analysis of RNA by Northern Blotting Using Riboprobes -- Rai Ajit K. Srivastava. -- RNA Quantitative Analysis form Fixed and Paraffin-Embedded Tissues -- Giorgio Stanta, Serena Bonin, and Ren ̌Utrera. -- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing -- Anu Suomalainen and Ann-Christine Syvñen. -- Preparation of Tissue Sections and Slides for mRNA Hybridization -- Giorgio Terenghi. -- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes -- Giorgio Terenghi . -- One-Tube RT-PCR with Sequence-Specific Primers -- Ulrich Pfeffer -- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA -- Thomas C. G. Bosch and Jan U. Lohmann. -- Characterization of RNA Using Continuous RT-PCR Coupled with ELISA -- Franȯis Mallet, Guy Oriol, and Bernard Mandrand. -- Gene Expression Analysis by CD-RT-PCR -- Eric de Kant. -- Primer Extension Analysis of mRNA -- Maggie Walmsley, Mark Leonard, and Roger Patient. -- S1 Mapping Using Single-Stranded DNA Probes -- Stp̌hane Viville and Roberto Mantovani. -- Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay -- Rai Ajit Srivastava and Gustav Schonfeld. -- Transcription In Vitro Using Bacteriophage RNA Polymerases -- Elaine T. Schenborn. -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- Louise Olliver and Charles D. Boyd. -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- Louse Olliver, Ann Grobler-Rabie, and Charles D. Boyd. -- The Xenopus Egg Extract Translation System -- Glenn M. Matthews and Alan Colman. -- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization -- Lesley-Ann Martin and Peter P. C. Mertens. -- Index |
Bibliography |
Includes bibliographical references and index |
Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
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Methods in molecular biology (Clifton, N.J.) no:86 1064-3745 |
Subject |
RNA -- Analysis -- Laboratory manuals.
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RNA -- Purification -- Laboratory manuals.
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Author |
Manning, David L.
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Rapley, Ralph.
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Humana Press.
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LC no. |
98012453 |
ISBN |
0896033937 (comb : alk. paper) |
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0896034941 (hard : alk. paper) |
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