| Description |
1 online resource (xiii, 260 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1680 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1680, 1064-3745
|
| Contents |
Cloning and identification of recombinant argonaute-bound small RNAs using next-generation sequencing / Pooja Gangras, Daniel M. Dayeh, Justin W. Mabin, Kotaro Nakanishi, and Guramrit Singh -- Quantification of miRNAs co-immunoprecipitated with argonaute proteins using SYBR green-based qRT-PCR / Hong-Duc Phan, Junan Li, Ming Poi, and Kotaro Nakanishi -- Gateway to understanding argonaute loading of single-stranded RNAs : preparation of deep sequencing libraries with in vitro loading samples / Eling Goh and Katsutomo Okamura -- Dumbbell-PCR for discriminative quantification of a small RNA variant / Megumi Shigematsu, Shozo Honda, and Yohei Kirino -- MicroRNA detection by whole-mount in situ hybridization in C. elegans / Yoshiki Andachi and Yuji Kohara -- cCLIP-seq : retrieval of chimeric reads from HITS-CLIP (CLIP-Seq) libraries / Panagiotis Alexiou, Manolis Maragkakis, Zissimos Mourelatos, and Anastassios Vourekas-- Kinetic analysis of small silencing RNA production by human and drosophila dicer enzymes in vitro / Susan E. Liao and Ryuya Fukunaga -- Nucleic acid-binding assay of argonaute protein using fluorescence polarization / Tomohiro Miyoshi -- Reconstitution of RNA interference machinery / Shintaro Iwasaki and Yukihide Tomari -- Single-molecule analysis for RISC assembly and target cleavage / Hiroshi M. Sasaki, Hisashi Tadakuma, and Yukihide Tomari -- Profiling open chromatin structure in the ovarian somatic cells using ATAC-seq / Kensaku Murano, Yuka W. Iwasaki, and Haruhiko Siomi -- Assessing miR-451 activity and its role in erythropoiesis / Dmitry A. Kretov, Andrew M. Shafik, and Daniel Cifuentes -- Functional analysis of microRNAs in neurogenesis during mouse cortical development / Wei Zhang, Xiaoxia Zeng, and Li Zeng -- Cellular approaches in investigating argonaute2-dependent RNA silencing / Cai Zhang, Joonbae Seo, and Takahisa Nakamura -- Genomic tagging of AGO1 using CRISPR/Cas9-mediated homologous recombination / Sanjay Ghosh and Ji-Long Liu -- Accurate profiling and quantification of tRNA fragments from RNA-Seq data : a Vade Mecum for MINTmap / Phillipe Loher, Aristeidis G. Telonis, and Isidore Rigoutsos |
| Summary |
This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed October 19, 2017) |
| Subject |
RNA-protein interactions -- Laboratory manuals
|
|
RNA interference -- Laboratory manuals
|
|
RNA interference.
|
|
Argonaute Proteins -- analysis
|
|
RNA Interference
|
|
Proteins.
|
|
Science -- Life Sciences -- Biochemistry.
|
|
RNA interference
|
|
RNA-protein interactions
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Okamura, Katsutomo, editor.
|
|
Nakanishi, Kotaro, editor.
|
| ISBN |
9781493973392 |
|
1493973398 |
|
