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Title The nuclear receptor superfamily : methods and protocols / edited by Iain J. McEwan
Edition Second edition
Published New York, NY : Humana Press, 2016


Description 1 online resource (x, 250 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1443
Methods in molecular biology (Clifton, N.J.) ; v. 1443. 1064-3745
Contents The Nuclear Receptor Superfamily- At Thirty -- Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization -- Use of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands -- Drug-Discovery Pipeline for Novel Inhibitors of the Androgen Receptor -- Use of BRET to Study Protein-Protein Interactions in vitro and in vivo -- Studying Steroid Receptor Complexes in the Cellular Environment -- Post-translational Modifications of Steroid Receptors: Phosphorylation -- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing -- Methods to Identify Chromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches -- Measuring the Expression of microRNAs Regulated by Androgens -- Methods for Identifying and Quantifying Mrna Expression of Androgen Receptor Splicing Variants in Prostate Cancer -- Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells -- In vivo Imaging of Nuclear Receptor Transcriptional Activity -- Development and Characterisation of Cell-Specific Androgen Receptor Knockout Mice
Summary This volume aims to describe a complementary range of molecular, cell biological, and in vivo protocols used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new drugs to selectively target nuclear receptor-associated diseases. The Nuclear Receptor Superfamily, Second Edition will benefit those starting out in the nuclear receptor research field as well as to more established researchers who wish to apply different methods to a particular receptor or research problem. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Nuclear Receptor Superfamily, Second Edition aims to ensure successful results in the further study of this vital field
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed June 10, 2016)
Subject Nuclear receptors (Biochemistry) -- Laboratory manuals
Receptors, Cytoplasmic and Nuclear -- physiology
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Nuclear receptors (Biochemistry)
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author McEwan, I. J., editor
ISBN 9781493937240