| Description |
1 online resource (xvii, 358 pages, 4 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in molecular biology ; 483 |
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Springer protocols |
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Springer protocols
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Methods in molecular biology (Clifton, N.J.) ; v. 483
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| Contents |
From Neanderthal to nanobiotech : from plant potions to pharming with plant factories / Christophe Sourrouille [and others] -- Cowpea mosaic virus-based systems for the expression of antigens and antibodies in plants / Frank Sainsbury, Li Liu, and George P. Lomonossoff -- Transient expression of antibodies in plants using syringe agroinfiltration / Marc-André D'Aoust [and others] -- Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants / Giuliana Medrano [and others] -- Production and localization of recombinant pharmaceuticals in transgenic seeds / Thomas Rademacher, Elsa Arcalis, and Eva Stoger -- Production of antibody fragments in Arabidopsis seeds / Bart Van Droogenbroeck, Kirsten De Wilde, and Ann Depicker -- Production of plantibodies in Nicotiana plants / Marta Ayala [and others] -- Physcomitrella patens : a non-vascular plant for recombinant protein production / David Liénard and Fabien Nogué -- Production of recombinant proteins in suspension-cultured plant cells / Carole Plasson [and others] -- Chloroplast-derived vaccine antigens and biopharmaceuticals : protocols for expression, purification, or oral delivery and functional evaluation / N. Dolendro Singh, Yi Ding, and Henry Daniell -- Protein body induction : a new tool to produce and recover recombinant proteins in plants / Margarita Torrent, Imma Llop-Tous, and M. Dolors Ludevid -- A case study for plant-made pharmaceuticals comparing different plant expression and production systems / Guy Vancanneyt [and others] -- Glycosylation of antibody therapeutics : optimisation for purpose / Roy Jefferis -- N-glycosylation of plant recombinant pharmaceuticals / Muriel Bardor [and others] -- Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts / Meriem Benchabane [and others] -- Strategies for improving vaccine antigens expression in transgenic plants : fusion to carrier sequences / Jose M. Escribano and Daniel M. Perez-Filgueira -- Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies / Manfred Gahrtz and Udo Conrad -- On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS / Amine M. Badri [and others] -- Assessing the risk of undesirable immunogenicity/allergenicity of plant-derived therapeutic proteins / Paul D. Chamberlain -- Biosafety, risk assessment, and regulation of plant-made pharmaceuticals / Penelope A.C. Sparrow and Richard M. Twyman |
| Summary |
The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Recombinant proteins.
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Plant proteins.
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Recombinant proteins -- Therapeutic use
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Plant genetic engineering.
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Plant gene expression.
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Plant genetics.
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Plant Proteins -- genetics
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Recombinant Proteins -- genetics
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Recombinant Proteins
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Plant Proteins
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Recombinant Proteins.
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Plant proteins.
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Recombinant Proteins -- therapeutic use.
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Plant genetic engineering.
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Plant gene expression.
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Protéines recombinantes.
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Protéines recombinantes -- Emploi en thérapeutique.
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Protéines végétales.
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Génie génétique végétal.
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Génétique végétale.
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Plant Proteins -- genetics.
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Recombinant Proteins -- genetics.
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Plant genetics
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Plant gene expression
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Plant genetic engineering
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Plant proteins
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Recombinant proteins
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Faye, Loïc.
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Gomord, Véronique.
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| ISBN |
9781588299789 |
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1588299783 |
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9781597454070 |
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1597454079 |
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