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Title Site-specific recombinases : methods and protocols / edited by Nikolai Eroshenko
Published New York : Humana Press : Springer, ©2017

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Description 1 online resource (xi, 342 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; v. 1642
Methods in molecular biology (Clifton, N.J.) ; v. 1642. 1064-3745
Contents Generating genetically modified mice : a decision guide / Ivo J. Huijbers -- Direct generation of conditional alleles using CRISPR/Cas9 in mouse zygotes / Colin E.J. Pritchard, Lona J. Kroese, and Ivo J. Huijbers -- Building cre knockin rat lines using CRISPR/Cas9 / Yuanwu Ma, Lianfeng Zhang, and Xingxu Huang -- Dual recombinase-mediated cassette exchange by tyrosine site-specific recombinases / Eugenia Voziyanova, Rachelle P. Anderson, and Yuri Voziyanov -- Use of the DICE (dual integrase cassette exchange) system / Alfonso P. Farruggio, Mital S. Bhakta, and Michele P. Calos -- Ligand-controlled site-specific recombination in zebrafish / Avinash Chekuru, Veronika Kuscha, Stefan Hans, and Michael Brand -- Injection-based delivery of cell-permeable peptide-tagged cre / Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, and Michael T. Chin -- Viral delivery of GFP-dependent recombinases to the mouse brain / Jonathan C.Y. Tang, Stephanie Rudolph, and Constance L. Cepko -- Recombinase-mediated cassette exchange using adenoviral vectors / Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, and David A. Sorrell -- Marker removal in transgenic plants using cre recombinase delivered with potato virus X / Lilya Kopertekh and Joachim Schiemann -- Nanoparticle-mediated recombinase delivery into maize / Susana Martin-Ortigosa, Brian G. Trewyn, and Kan Wang -- Immunohistochemical procedures for characterizing the retinal expression patterns of cre driver mouse lines / Qi Lu and Zhuo-Hua Pan -- FLPing genes on and off in Drosophila / Bonnie M. Weasner, Jinjin Zhu, and Justin P. Kumar -- Imaging neural architecture in brainbow samples / Douglas H. Roossien and Dawen Cai -- Purification and in vitro characterization of zinc finger recombinases / Femi J. Olorunniji, Susan J. Rosser, and W. Marshall Stark -- Preparing mate-paired illumina libraries using cre recombinase / Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, and Feng Chen -- Preparing fosmid mate-paired libraries using Cre-LoxP recombination / Ze Peng, Jeff L. Froula, and Jan-Fang Cheng -- Using purified tyrosine site-specific recombinases in vitro to rapidly construct and diversify metabolic pathways / Wei Liu, Laura R. Tuck, Jon Marles Wright, and Yizhi Cai -- Multipart DNA assembly using site-specific recombinases from the large serine integrase family / Femi J. Olorunniji, Christine Merrick, Susan J. Rosser, Margaret C.M. Smith, W. Marshall Stark, and Sean D. Colloms -- Production of minicircle DNA vectors using site-specific recombinases / Nafiseh Sanei Ata-abadi, Naeimeh Rezaei, Kianoush Dormiani, and Mohammad Hossein Nasr-Esfahani
Summary This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed August 17, 2017)
Subject Genetic recombination -- Laboratory manuals
Recombinant DNA -- Laboratory manuals
Gene editing.
Genetic Engineering -- methods
Recombinases
Gene Editing
Medical genetics.
Medical -- Genetics.
Gene editing
Genetic recombination
Recombinant DNA
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Eroshenko, Nikolai, editor.
ISBN 9781493971695
1493971697
1493971670
9781493971671