| Description |
1 online resource (xii, 232 pages) : illustrations |
| Contents |
Contents -- A Synthetic Peptide Libraries -- 1 Soluble Synthetic Combinatorial Libraries: The Use of Molecular Diversities for Drug Discovery -- 1.1 Introduction -- 1.2 Synthetic Combinatorial Libraries (SCLs) -- 1.3 Synthetic Methods for the Generation of SCLs -- 1.4 SCLs in Drug Discovery and Basic Research -- 1.5 Conclusion -- 2 Combinatorial Libraries of Synthetic Structures: Synthesis, Screening, and Structure Determination -- 2.1 Introduction -- 2.2 One-Bead One-Structure Concept -- 2.3 Design and Synthesis of Non-Peptide Libraries -- 2.4 Release Assay |
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2.5 Stucture Determination2.6 Conclusion -- 3 Peptide Libraries Bound to Continuous Cellulose Membranes: Tools to study Molecular Recognition -- 3.1 Introduction -- 3.2 Detection of antibody epitopes -- 3.3 Mutational analyses of peptide epitopes -- 3.4 Positional scanning combinatorial library -- 3.5 Indentification of metal binding peptides -- 3.6 Summary -- B Nucleic Acids Libraries -- 4 In Vitro Selection of Nucleic Acid Sequences that Bind Small Molecules -- 4.1 Introduction -- 4.2 Natural RNA Receptors -- 4.3 In Vitro Selection |
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4.4 Amino Acid Aptamers4.5 Cofactors -- 4.6 DNA Aptamers -- 4.7 The Complexity of Complexity -- 4.8 Aptamer Structures -- 4.9 Transition State Stabilization and Tight Binding -- 4.10 Conclusions -- 5 Discovery and Characterization of a Thrombin Aptamer Selected from a Combinatorial ssDNA Library -- 5.1 Introduction -- 5.2 Discovery and Initial Characterization -- 5.3 Structure -- 5.4 Binding Site on Thrombin -- 5.5 Determination of Kd and Ki -- 5.6 In vitro Activity -- 5.7 In Vitro Activity -- 5.8 Conclusions -- C Phage Display of Peptide Libraries |
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6 Structural and Functional Constraints in the Display of Peptides on Filamentous Phage Capsids6.1 Introduction -- 6.2 The Phage Life Cycle -- 6.3 A Low Resolution Model -- 6.4 Extending the Amino-Terminus of pVIII -- 6.5 Modifying the Surface of Filamentous Phage by Amino Acid Substitution -- 6.6 Can the Remaining Minor Proteins Support Modification? -- 7 Conformationally Defined Peptide Libraries on Phage: Selectable Templates for the Design of Pharmacological Agents -- 7.1 Introduction -- 7.2 From Peptides to Peptidomimetics |
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7.3 Building Constraints in Phage-Displayed Polypeptides7.4 The Minibody: An Engineered ß-Pleated Scaffold for the Display of Reverse-Turn Motifs -- 7.5 The Zinc Finger: A Small Domain for the Display of Structurally Homogeneous α-Helical Motifs -- 7.6 Future Developments: Progressing Toward Non-Peptide Pharmaceuticals -- 8 Discovery of Disease-Specific Mimotopes by Screening Phage Libraries with Human Serum Samples -- 8.1 Introduction -- 8.2 Using Polyclonal Antibodies as a Ligate for the Selection of RPL |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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In English |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
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Print version record |
| In |
Druckausg.: Combinatorial libraries |
| Subject |
Nucleotide sequence.
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Amino acid sequence.
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Genetic vectors.
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Base Sequence
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Amino Acid Sequence
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Genetic Vectors
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SCIENCE -- Life Sciences -- Biology.
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Amino acid sequence.
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Genetic vectors.
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Nucleotide sequence.
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Séquence nucléotidique.
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Vecteurs de clonage.
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Séquençage des acides aminés.
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| Form |
Electronic book
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| Author |
Cortese, Riccardo
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| ISBN |
9783110808902 |
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3110808900 |
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