Title Protein sequencing protocols / edited by Bryan John Smith

Edition 2nd ed Published Totowa, N.J. : Humana Press, ©2002

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Description 1 online resource (xii, 493 pages) : illustrations Series Methods in molecular biology ; v. 211 Methods in molecular biology (Clifton, N.J.) ; volume 211. Contents Preface -- Contributors -- 1 Strategies for Handling Polypeptides on a Micro-Scale -- Bryan John Smith and Paul Tempst -- 2 SDS Polyacrylamide Gel Electrophoresis -- for N-Terminal Protein Sequencing -- Bryan John Smith -- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis -- for the Separation of Proteins for Chemical Characterization -- Michael J. Dunn -- 4 Electroblotting of Proteins from Polyacrylamide Gels -- for Chemical Characterization -- Michael J. Dunn -- 5 Enzymatic Cleavage of Proteins -- Bryan John Smith -- 6 Chemical Cleavage of Polypeptides -- Bryan John Smith -- 7 Enzymatic Preparation and Isolation of Glycopeptides -- David J. Harvey -- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Alan J. Smith -- 9 High-Performance Liquid Chromatography On-Line Derivative -- Spectroscopy for the Characterization of Peptides -- with Aromatic Amino Acid Residues -- Christoph W. Turck -- 10 Hydrolysis of Samples for Amino Acid Analysis -- lan Davidson -- 11 Amino Acid Analysis: Precolumn Derivatization Methods -- G. Brent Irvine and lan Davidson -- 12 Post Column Amino Acid Analysis -- Alan J. Smith -- 13 Amino Acid Analysis Using Pre-Column Derivatization -- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: -- Analysis of Hydrolyzed Proteins and Electroblotted Samples -- Steven A. Cohen -- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange -- Chromatography and IPAD Detection -- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic -- 15 D-Amino Acid Analysis -- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa -- 16 Validation of Amino Acid Analysis Methods -- Andrew J. Reason -- 17 Electrospray Mass Spectrometry of Peptides, Proteins -- and Glycoproteins -- Fiona M. Greer and Howard R. Morris -- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Darryl J.C. Pappin -- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence -- Similarity Searching for Identification of Proteins -- from Organisms with Unknown Genomes -- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, -- and Andrej Shevchenko -- 20 Direct Identification of Proteins in Ultracomplex Mixtures: -- Applications to Proteome Analysis -- David M. Schieltz and John R. Yates, 111 -- 21 Identification of PTH-Amino Acids by HPLC -- Gregory A. Grant and Mark W. Crankshaw -- 22 Protein Sequencer Maintenance and Troubleshooting -- Bryan Dunbar -- 23 N-Terminal Protein Sequencing for Special Applications -- Philip J. Jackson -- 24 Identification of Phosphorylation Sites by Edman Degradation -- Laurey Steinke and Richard G. Cook -- 25 Validation of Protein Sequencing in a Regulated Laboratory -- Philip J. Jackson and Neil Dodsworth -- 26 Automated C-Terminal Protein Sequence Analysis -- Using the Alkylated-Thiohydantoin Method -- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham -- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Alex F. Carne -- 28 Deblocking of N-Terminally Modified Proteins -- Hisashi Hirano and Roza M. Kamp -- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- Jacek Mozdzanowski -- 30 Identification of Sites of Glycosylation -- David J. Harvey -- 31 Analysis of Sites of Protein Phosphorylation -- Alastair Aitken and Michele Learmonth -- 32 Quantitation and Location of Disulfide Bonds in Proteins -- Alastair Aitken and Michele Learmonth -- 33 Getting the Most from Your Protein Sequence -- Richard R. Copley and Robert B. Russell -- Appendix 1: Letter Codes, Structures, Masses, and Derivatives -- of Amino Acids -- James P. Turner -- Appendix 2: Protein Consensus Sequence Motifs -- Alastair Aitken Summary Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Nucleotide sequence -- Laboratory manuals Genetics -- Technique. Documentation. Information services. Molecular structure. Proteins. Molecular Sequence Data Amino Acids, Peptides, and Proteins Genetic Structures Sequence Analysis Chemicals and Drugs Biochemical Phenomena Genetic Phenomena Genetic Techniques Documentation Information Services Investigative Techniques Phenomena and Processes Chemical Phenomena Analytical, Diagnostic and Therapeutic Techniques and Equipment Information Science Molecular Structure Sequence Analysis, Protein Proteins Base Sequence documentation (activity) molecular structure. protein. information services. documentation centers. SCIENCE -- Life Sciences -- Genetics & Genomics. Proteins Molecular structure Information services Genetics -- Technique Documentation Nucleotide sequence Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Smith, Bryan John ISBN 9781592593422 1592593429 0896039757 9780896039759 1280842253 9781280842252 9786610842254 6610842256

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