Description |
1 online resource (xiii, 262 pages) : illustrations (some color) |
Series |
Methods in molecular biology, 1940-6029 ; 1237 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1237. 1064-3745
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Contents |
Microbiological diagnosis of sepsis : the confounding effects of a "Gold Standard" / Nicasio Mancini, Roberto Burioni, and Massimo Clementi -- Pathophysiological aspects of sepsis : an overview / Yong-Ming Yao [and others] -- Clinical aspects of sepsis : an overview / Giacomo Monti [and others] -- Technical improvements in culturing blood / Giacomo Pardini -- Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS)-based identification of pathogens from positive blood culture bottles / Philippe Lagacé-Wiens -- Bacterial and fungal DNA extraction from positive blood culture bottles : a manual and an automated protocol / Minna Mäki -- Broad-range PCR in the identification of bacterial and fungal pathogens from positive blood culture bottles : a sequencing approach / Yoshitomo Morinaga and Katsunori Yanagihara -- Identification of bacterial and fungal pathogens from positive blood culture bottles : a microarray-based approach / Teresa Raich and Scott Powell -- Detection of carbapenemases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) meropenem hydrolysis assay / Jaroslav Hrabák -- Molecular detection of antibiotic resistance genes from positive blood cultures / Musa Y. Hindiyeh -- Bacterial and fungal DNA extraction from blood samples : manual protocols / Michael G. Lorenz, Helge Mühl, and Claudia Disqué -- Bacterial and fungal DNA extraction from blood samples : automated protocols / Michael G. Lorenz, Claudia Disqué, and Helge Mühl -- Broad-range PCR for the identification of bacterial and fungal pathogens from blood : a sequencing approach / Eva Leitner and Harald H. Kessler -- Real-time PCR-based identification of bacterial and fungal pathogens from blood samples / Madeleine Mai [and others] -- Host response biomarkers in the diagnosis of sepsis : a general overview / Marianna Parlato and Jean-Marc Cavaillon -- Host response biomarkers in sepsis : the role of procalcitonin / Jean-Louis Vincent, Marc Van Nuffelen, and Christophe Lelubre -- Host response biomarkers in sepsis : overview on sTREM-1 detection / Jérémie Lemarié, Damien Barraud, and Sébastien Gibot -- Host response biomarker in sepsis: suPAR detection / Evangelos J. Giamarellos-Bourboulis and Marianna Georgitsi -- Clinical diagnosis of sepsis and the combined use of biomarkers and culture- and non-culture-based assays / Frank Bloos |
Summary |
This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness |
Analysis |
microbiologie |
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microbiology |
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pathologie |
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pathology |
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sepsis |
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Medicine (General) |
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Geneeskunde (algemeen) |
Bibliography |
Includes bibliographical references and index |
Notes |
Print version record |
Subject |
Septicemia.
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Sepsis -- diagnosis
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Sepsis -- microbiology
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Sepsis
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Pathology.
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Microbiology (non-medical)
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Medical -- Pathology.
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Science -- Life Sciences -- Biology -- Microbiology.
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Septicemia
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Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Mancini, Nicasio, editor
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ISBN |
9781493917761 |
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1493917765 |
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1493917757 |
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9781493917754 |
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