| Description |
1 online resource (xxvi, 198 pages) : color illustrations |
| Contents |
Machine generated contents note: 1. Pipetting -- 2. Serology -- 3. Sampling Biological Evidence for DNA Extraction -- 4. DNA Extraction -- 5. Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis -- 6. Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy -- 7. Determination of DNA Quantity by Fluorescence Spectroscopy -- 8. Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA -- 9. Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico) -- 10. Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions -- 11. Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit -- 12. Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex Kit -- 13. Computing Random Match Probability from DNA Profile Data Using Population Databases -- 14. Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection -- 15. Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit -- 16. Ribonucleic Acid (RNA) Extraction -- 17. Y-STR Polymerase Chain Reaction (PCR) Deoxyribonucleic acid (DNA) Amplification and Typing -- 18. Human Genetic Analysis: Paternity or Missing Persons Cases and Statistics -- 19. Low Copy Number Stochastic Results -- 20. Using in Silico Methods to Construct a Short-Tandem Repeat (STR) Deoxyribonucleic Acid (DNA) Sequence for Cloning -- 21. Deoxyribonucleic Acid (DNA) Extraction from Botanical Material and Polymerase Chain Reaction (PCR) Amplification -- 22. Social, Ethical, and Regulatory Concerns |
| Summary |
DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout and the author will provide an Instructor Manual and PowerPoint lecture slides. Provides practical step-by-step instruction on how to perform forensic DNA analysis. Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics. Covers techniques such as pipetting, microscopy and DNA extraction. Pre- and post-lab exercises and questions assist the reader in learning the material. Report writing templates assure the reader learns real world crime lab procedure |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Forensic genetics.
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DNA fingerprinting.
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Polymerase chain reaction.
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DNA Fingerprinting
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Forensic Genetics -- methods
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Polymerase Chain Reaction
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Sequence Analysis, DNA -- methods
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Polymerase chain reaction
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DNA fingerprinting
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Forensic genetics
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| Form |
Electronic book
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| LC no. |
2012554282 |
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