| Description |
1 online resource (xvi, 551 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1619 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1619. 1064-3745
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| Contents |
Direct assessment of plasma/serum sample quality for proteomics biomarker investigation / Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani -- Protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies / Rosemary L. Sparrow, Richard J. Simpson, and David W. Greening -- Preparation of platelet concentrates for research and transfusion purposes / David W. Greening, Richard J. Simpson, and Rosemary L. Sparrow -- Bead-based and multiplexed immunoassays for protein profiling via sequential affinity capture / Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu -- Affinity proteomics for fast, sensitive, quantitative analysis of proteins in plasma / John P. O'Grady, Kevin W. Meyer, and Derrick N. Poe -- Characterization of the low-molecular-weight human plasma peptidome / David W. Greening and Richard J. Simpson -- In-depth, reproducible analysis of human plasma using IgY 14 and supermix immunodepletion / Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher -- Low-molecular-weight plasma proteome analysis using top-down mass spectrometry / Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, and Ji Eun Lee -- Identification of post-translational modifications from serum/plasma by immunoaffinity enrichment and LC-MS/MS analysis without depletion of abundant proteins / Hongbo Gu, Jianmin Ren, Xiaoying Jia, and Matthew P. Stokes -- Identification of core-fucosylated glycoproteome in human plasma / Qichen Cao, Qing Zhao, Xiaohong Qian, and Wantao Ying -- Proteomic analysis of blood extracellular vesicles in cardiovascular disease by LC-MS/MS analysis / Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, and Maria G. Barderas -- Targeted approach for proteomic analysis of a hidden membrane protein / Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, and Henrique Girao -- Red blood cells in clinical proteomics / Ana Sofia Carvalho, Manuel S. Rodriguez, and Rune Matthiesen -- High-throughput quantitative lipidomics analysis of nonesterified fatty acids in plasma by LC-MS / Nicolas Christinat, Delphine Morin-Rivron, and Mojgan Masoodi -- Simultaneous enrichment of plasma extracellular vesicles and glycoproteome for studying disease biomarkers / Sunil S. Adav and Siu Kwan Sze -- Lipidomics of human blood plasma by high-resolution shotgun mass spectrometry / Susanne Sales, Oskar Knittelfelder, and Andrej Shevchenko -- Proteomics analysis of circulating serum exosomes / Antonius Koller, Purvi Patel, Jenny Kim Kim, and Emily I. Chen -- High-density serum/plasma reverse phase protein arrays / Cecilia Hellström, Tea Dodig-Crnković, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, and Ronald Sjöberg -- Antibody colocalization microarray for cross-reactivity-free multiplexed protein analysis / Véronique Laforte, Pik-Shan Lo, Huiyan Li, and David Juncker -- Surface profiling of extracellular vesicles from plasma or ascites fluid using DotScan antibody microarrays / Larissa Belov, Susannah Hallal, Kieran Matic, Jerry Zhou, Sandra Wissmueller, Nuzhat Ahmed, Sumaiya Tanjil, Stephen P. Mulligan, O. Giles Best, Richard J. Simpson, and Richard I. Christopherson -- Serum profiling for identification of autoantibody signatures in diseases using protein microarrays / Shabarni Gupta, K.P. Manubhai, Shuvolina Mukherjee, and Sanjeeva Srivastava -- Quantitative comparisons of large numbers of human plasma samples using TMT10plex labeling / Pengyuan Liu, Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher -- Efficient quantitative comparisons of plasma proteomes using label-free analysis with MaxQuant / Lynn A. Beer, Pengyuan Liu, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher -- Blood and plasma proteomics : targeted quantitation and posttranslational redox modifications / Julie A. Reisz, Katelyn M. Chessler, Monika Dzieciatkowska, Angelo D'Alessandro, and Kirk C. Hansen -- SWATH mass spectrometry for proteomics of non-depleted plasma / Christoph Krisp and Mark P. Molloy -- Shotgun and targeted plasma proteomics to predict prognosis of non-small cell lung cancer / Qing-Run Li, Yan-Sheng Liu, and Rong Zeng -- High-throughput parallel proteomic sample preparation using 96-well polyvinylidene fluoride (PVDF) membranes and C18 purification plates / Tue Bjerg Bennike and Hanno Steen -- Targeted auantification of the glycated peptides of human serum albumin / Garikapati Vannuruswamy, Arvind M. Korwar, Mashanipalya G. Jagadeeshaprasad, and Mahesh J. Kulkarni -- Absolute quantification of middle- to high-abundant plasma proteins via targeted proteomics / Julia Dittrich and Uta Ceglarek -- Highly automated shotgun proteomic workflow : clinical scale and robustness for biomarker discovery in blood / Loïc Dayon, Antonio Núñez Galindo, Ornella Cominetti, John Corthésy, and Martin Kussmann -- Mass spectrometry-based serum proteomics for biomarker discovery and validation / Santosh D. Bhosale, Robert Moulder, Petri Kouvonen, Riitta Lahesmaa, and David R. Goodlett -- Metabolomics toward biomarker discovery / Peiyuan Yin and Guowang Xu -- Plasma biomarker identification and quantification by microparticle proteomics / Michal Harel and Tamar Geiger -- Bronchoalveolar lavage : quantitative mass spectrometry-based proteomics analysis in lung diseases / Ana Sofia Carvalho and Rune Matthiesen -- Protein multiplexed immunoassay analysis with R / Edmond J. Breen -- Appendix A Standard operating procedures for plasma collection in clinical research -- Appendix B Standard operating procedures for serum collection in clinical research -- Appendix C Reference ranges for blood tests are sorted by mass and molarity |
| Summary |
Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed July 5, 2017) |
| Subject |
Blood plasma -- Laboratory manuals
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Serum -- Laboratory manuals
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Blood plasma.
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Serum.
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Blood Proteins -- analysis
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Proteomics -- methods
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Biomarkers -- blood
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Plasma
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Serum
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Blood plasma
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Serum
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Simpson, Richard J., editor
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Greening, David W., editor
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| LC no. |
2017944282 |
| ISBN |
9781493970575 |
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1493970577 |
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9781493970568 |
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1493970569 |
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