| Description |
1 online resource (xvii, 554 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 927 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 927. 1064-3745
|
| Contents |
Methods for sperm concentration determination / Lars Bjorndahl -- Methods of sperm vitality assessment / Sergey I. Moskovtsev and Clifford L. Librach -- Hypo-osmotic swelling test for evaluation of sperm membrane integrity / Sivakumar Ramu and Rajasingam S. Jeyendran -- Sperm morphology classification : a rational method for schemes adopted by the World Health Organization / Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow -- Sperm morphology assessment using strict (tygerberg) criteria / Roelof Menkveld -- Methods for direct and indirect antisperm antibody testing / Hiroaki Shibahara and Junko Koriyama -- Manual methods for sperm motility assessment / David Mortimer and Sharon T. Mortimer -- Computer-aided sperm analysis (CASA) of sperm motility and hyperactivation / David Mortimer and Sharon T. Mortimer -- Hemizona assay for assessment of sperm function / Sergio Oehninger, Mahmood Morshedi, and Daniel Franken -- Sperm penetration assay for the assessment of fertilization capacity / Kathleen Hwang and Dolores J. Lamb -- Methods for the assessment of sperm capacitation and acrosome reaction excluding the sperm penetration assay / Christopher J. De Jonge and Christopher L.R. Barratt -- Sperm DNA fragmentation analysis using the TUNEL assay / Rakesh Sharma, Jayson Masaki, and Ashok Agarwal -- Sperm DNA damage measured by comet assay / Luke Simon and Douglas T. Carrell -- Sperm chromatin structure assay (SCSA) / Donald P. Evenson -- Sperm aneuploidy testing using fluorescence in situ hybridization / Benjamin R. Emery -- Flow cytometric methods for sperm assessment / Vanesa Robles and Felipe Martinez-Pastor -- Human y chromosome microdeletion analysis by PCR multiplex protocols identifying only clinically relevant AZF microdeletions / Peter H. Vogt and Ulrike Bender -- Sperm cryopreservation methods / Tiffany Justice and Greg Christensen -- Density gradient separation of sperm for artificial insemination / David Mortimer and Sharon T. Mortimer -- Recovery, isolation, identification, and preparation of spermatozoa from human testis / Charles H. Muller and Erin R. Pagel -- Enhancement of sperm motility using pentoxifylline and platelet-activating factor / Shilo L. Archer and William E. Roudebush -- Intracytoplasmic morphology-selected sperm injection / Luke Simon, Aaron Wilcox, and Douglas T. Carrell -- Sperm selection for ICSI using annexin V / Sonja Grunewald and Uwe Paasch -- Sperm selection for ICSI using the hyaluronic acid binding assay / Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh -- sperm selection based on electrostatic charge / Luke Simon, Shao-qin Ge, and Douglas T. Carrell -- Sex-sorting sperm using flow cytometry/cell sorting / Duane L. Garner, K. Michael Evans, and George E. Seidel -- Assessment of spermatogenesis through staging of seminiferous tubules / Marvin L. Meistrich and Rex A. Hess -- Immunohistochemical approaches for the study of spermatogenesis / Cathryn A. Hogarth and Michael D. Griswold -- Ultrastructural analysis of testicular tissue and sperm by transmission and scanning electron microscopy / Hector E. Chemes -- Assessment of oxidative stress in sperm and semen / Anthony H. Kashou, Rakesh Sharma, and Ashok Agarwal -- Improved chemiluminescence assay for measuring antioxidant capacity of seminal plasma / Charles H. Muller, Tiffany K.Y. Lee, and Michalina A. Montano -- Methods of sperm DNA extraction for genetic and epigenetic studies / Jeanine Griffin -- Isolating mRNA and small noncoding RNAs from human sperm / Robert J. Goodrich, Ester Anton, and Stephen A. Krawetz -- Review of genome-wide approaches to study the genetic basis for spermatogenic defects / Kenneth I. Aston and Donald F. Conrad -- Methods for the analysis of the sperm proteome / Sara de Mateo, Josep Maria Estanyol, and Rafael Oliva -- Methodology of aniline blue staining of chromatin and the assessment of the associated nuclear and cytoplasmic attributes in human sperm / Leyla Sati and Gabor Huszar -- Isolation of sperm nuclei and nuclear matrices from the mouse, and other Rodents / W. Steven Ward -- Protamine extraction and analysis of human sperm protamine 1/protamine 2 ratio using acid gel electrophoresis / Lihua Liu, Kenneth I. Aston, and Douglas T. Carrell -- Analysis of gene-specific and genome-wide sperm DNA methylation / Saher Sue Hammoud, Bradley R. Cairns, and Douglas T. Carrell -- Evaluating the localization and DNA binding complexity of histones in mature sperm / David Miller and Agnieszka Paradowska -- In vitro spermatogenesis using an organ culture technique / Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, and Takehiko Ogawa -- Testicular tissue grafting and male germ cell transplantation / Jose R. Rodriguez-Sosa, Lin Tang, and Ina Dobrinski -- Transgenic modification of spermatogonial stem cells using lentiviral vectors / Christina Tenenhaus Dann -- Methods for sperm-mediated gene transfer / Marialuisa Lavitrano, Roberto Giovannoni, and Maria Grazia Cerrito -- Phenotypic sssessment of male fertility status in transgenic animal models / David M. de Kretser and Liza O'Donnell |
| Summary |
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike |
| Analysis |
Life sciences |
|
Cytology |
|
Developmental biology |
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Embryology |
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Cell Biology |
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spermatogenese |
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spermatogenesis |
|
celbiologie |
|
cellular biology |
|
mechatronica |
|
mechatronics |
|
ontogenie |
|
ontogeny |
|
gametogenese |
|
gametogenesis |
|
Ontogeny, Embryology |
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Ontogenie, embryologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Online resource; title from PDF title page (Springer Protocols, viewed October 30, 2012) |
| Subject |
Spermatogenesis -- Laboratory manuals
|
|
Spermatogenesis.
|
|
Spermatogenesis
|
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Spermatogenesis
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Carrell, Douglas T.
|
|
Aston, Kenneth I.
|
| ISBN |
9781627030380 |
|
1627030387 |
|
