| Description |
1 online resource (106 pages) |
| Contents |
Intro; CONTENTS; FOREWORD; PREFACE; ABBREVIATIONS; ABBREVIATIONS; Basics of In Vitro Cell Culture; INTRODUCTION; 1.1. Tissue Culture and Its Types; 1.2. Cell Line; 1.3. Types of Culture; 1.4. Equipments for Cell Culture Lab; 1.5. Materials for Cell Culture; 1.6. Cryopreservation of Cell Lines; 1.7. Cell Line Authentication; 1.8. Contamination in Cell Lines; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Methods of Transfection; INTRODUCTION; 2.1. Chemical Methods of Transfection; 2.2. Physical Methods of Gene Delivery |
|
2.3. Biological Methods of TransfectionCONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Apoptosis; INTRODUCTION; 3.1. Mediators of Apoptosis; 3.2. Activation of Apoptosis; 3.3. Methods for Detecting Apoptosis; 3.3.1. Analysing Cytomorphological Alterations; 3.3.2. DNA Fragmentation for Detecting Apoptosis; 3.3.3. Mitochondrial Markers for Detecting Apoptosis; 3.3.4. Measuring Changes in Cell Membrane to Detect Apoptosis; 3.3.5. Measuring Caspase Activity for the Detection of Apoptosis; CONCLUDING REMARKS; CONSENT FOR PUBLICATION |
|
CONFLICT OF INTERESTACKNOWLEDGEMENTS; REFERENCES; Cell Cytotoxicity, Viability and Proliferation; INTRODUCTION; 4.1. Trypan Blue Assay; 4.2. Neutral Red (3-Amino-7-Dimethyamino-2-Methylphenazine Hydrochlo- ride); 4.3. Calcein AM; 4.4. CFSE; 4.5. Sulphorhodamine B Assay; 4.6. Assays Based on Metabolic Activity; 4.7. Lactate Dehydrogenase Assay; 4.8. ATP Bioluminescent Assays; 4.9. Clonogenic Assay; 4.10. DNA Synthesis as a Marker for Cell Proliferation; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Reactive Oxygen Species (ROS); INTRODUCTION |
|
5.1. Detection of Lipid Peroxidation5.2. Glutathione Assay; 5.3. Superoxide Assay; 5.4. Nitric Oxide Detection Assay; 5.5. Hydrogen Peroxide Detection Assay; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Protein-Protein Interactions; INTRODUCTION; 6.1. Co-Immunoprecipitation; 6.2. Pull Down Assays; 6.3. Far-Western Blotting; CONCLUSION; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Protein-Nucleic Acid Interactions; INTRODUCTION; 7.1. Electrophoretic Mobility Shift Assay (EMSA) |
|
7.2. Chromatin Immunoprecipitation (ChIP) Assay7.3. Affinities Capture Methods; 7.4. Pull-Down Methods; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; GLOSSARY; SUBJECT INDEX |
| Notes |
Print version record |
| Subject |
Stem cells -- Research.
|
|
Embryonic stem cells -- Research.
|
|
Embryonic stem cells -- Research
|
|
Stem cells -- Research
|
| Form |
Electronic book
|
| Author |
Balkhi, Henah Mehraj
|
|
Haq, Ehtishamul
|
| ISBN |
1681087030 |
|
9781681087030 |
|
