| Description |
1 online resource (x, 351 pages) : illustrations (some color) |
| Series |
Methods in molecular medicine, 1543-1894 ; 104 |
|
Methods in molecular medicine ; 104. 1543-1894
|
| Contents |
Introduction to stroke genomics / Simon J. Read and Frank C. Barone -- Choice, methodology, and characterization of ischemic stroke models : the search for clinical relevance / David Virley -- Mutant animal models of stroke and gene expression : the stroke prone spontaneously hypertensive rat / Hilary V.O. Carswell [and others] -- Gene therapy in neurological disease / Midori A. Yenari and Robert M. Sapolsky -- Stem cell transplantation after middle cerebral artery occlusion / Paul Stroemer and Helen Hodges -- Endogenous brain protection : models, gene expression, and mechanisms / Frank C. Barone -- Production of transgenic and mutant mouse models / Alex Harper |
|
3-nitropropionic acid model of metabolic stress : assessment by magnetic resonance imaging / Toby John Roberts -- Practicalities of genetic studies in human stroke / Ahamad Hassan and Hugh S. Markus -- Evaluation of the interactions of common genetic mutations in stroke / Zoltán Szolnoki -- Technologies of disease-related gene discovery using preclinical models of stroke / Xinkang Wang -- Quantitative analysis of gene transcription in stroke models using real-time RT-PCR / David C. Harrison and Brian C. Bond -- Effective analysis of genomic data / Paul R. Nelson, Andrew B. Goulter, and Richard J. Davis -- Bioinformatic approaches to assigning protein function from novel sequence data / David Michalovich and Richard Fagan -- Pragmatic target discovery from novel gene to functionally defined drug target : the interleukin-1 story / Stuart McRae Allen |
| Summary |
"Although numerous genomic studies of stroke have examined such factors as stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery, much work to determine the precise role of gene function in human neurological pathophysiology remains ahead. In Stroke Genomics: Methods and Reviews, leading experts in the field explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. Providing the reader with cutting-edge reviews of clinical and preclinical genomics, the authors relate changes in gene expression to physiological end points-such as functional imaging paradigms-to produce a more holistic approach in which molecular biology goes hand in hand with stroke pathophysiology. Topics of special interest include stem cell transplantation, gene therapy, clinical gene/gene interaction studies, and cytokine drug discovery. Readily reproducible techniques are provided, along with ancillary methods (imaging, animal models, human genetic study design, and transgenic animal production) that can be effectively applied to gene discovery. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Stroke Genomics: Methods and Reviews shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke, work that will greatly advance its understanding, as well as rapid development of the next generation of stroke therapeutics. Book jacket."--Jacket |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| In |
Springer Protocols |
| Subject |
Cerebrovascular disease -- Genetic aspects
|
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Cerebrovascular disease -- Molecular aspects
|
|
Genomics.
|
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Stroke -- genetics
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Genomics
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MEDICAL -- Neurology.
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Genomics
|
| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Read, Simon J.
|
|
Virley, David.
|
| ISBN |
1588293335 |
|
9781588293336 |
|
1592598366 |
|
9781592598366 |
|
1280359757 |
|
9781280359750 |
|
9786610359752 |
|
661035975X |
|
