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Title Epigenetics protocols / edited by Trygve O. Tollefsbol
Published Totowa, N.J. : Humana Press, ©2004


Description 1 online resource (xi, 302 pages) : illustrations
Series Methods in molecular biology ; 287
Methods in molecular biology (Clifton, N.J.) ; v. 287
Contents Methods of epigenetic analysis / Trygve O. Tollefsbol -- Chromatin immunoprecipitation assays / Yan Yan, Haobin Chen, and Max Costa -- Native chromatin immunoprecipitation / Alan W. Thorne, Fiona A. Myers, and Tim R. Hebbes -- Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation / Ryan A. Irvine and Chin-Lin Hsieh -- Restriction endonuclease accessibility as a determinant of altered chromatin structure / William M. Hempel and Pierre Ferrier -- Measuring changes in chromatin using micrococcal nuclease / Nicolas Steward and Hiroshi Sano -- DNasel hypersensitivity analysis of chromatin structure / Qianjin Lu and Bruce Richardson -- Inhibition of histone deacetylases / Cheng Liu and Dawei Xu -- Site-specific analysis of histone methylation and acetylation / David Umlauf, Yuji Goto, and Robert Feil -- Analysis of mammalian telomere position effect / Joseph A. Baur, Woodring E. Wright, and Jerry W. Shay -- Activity assays for poly-ADP ribose polymerase 1 / Eva Kirsten [and others] -- Multigenerational selection and detection of altered histone acetylation and methylation patterns: toward a quantitative epigenetics in Drosophila / Mark D. Garfinkel [and others]
Profiling DNA methylation by bisulfite genomic sequencing: problems and solutions / Liang Liu [and others] -- Methylation-sensitive single-strand conformation analysis: a rapid method to screen for and analyze DNA methylation / Jean Benhattar and Geneviève Clément -- SIRPH analysis, SNuPE with IP-RP-HPLC for quantitative measurements of DNA methylation at specific CpG sites / Osman El-Maarri -- Real-time PCR-based assay for quantitative determination of methylation status / Ulrich Lehmann and Hans Kreipe -- Denaturing gradient gel electrophoresis to detect methylation changes in DNA / Masahiko Shiraishi -- Photocrosslinking oligonucleotide hybridization assay for concurrent gene dosage and CpG methylation analysis / Risa Peoples, Michael Wood, and Reuel Van Atta -- Methylation-specific oligonucleotide microarray / Pearlly S. Yan, Susan H. Wei, and Tim Hui-Ming Huang -- Methylation-specific PCR in situ hybridization / Gerard J. Nuovo -- Relative quantitation of DNA methyltransferase mRNA by real-time RT-PCR assay / John Attwood and Bruce Richardson -- DMB (DNMT-magnetic beads) assay: measuring DNA methyltransferase activity in vitro / Tomoki Yokochi and Keith D. Robertson
Summary Recent advances in epigenetic research as well as the development of exciting new technologies have helped greatly in unraveling the many mysteries of nontraditional genetic processes. In Epigenetics Protocols, hands-on researchers describe state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Epigenetics Protocols offers investigators readily reproducible techniques that will further promote progress in this critically important field
Analysis epigenetica
moleculaire genetica
molecular genetics
biologische technieken
biological techniques
Molecular Genetics
Moleculaire genetica
Bibliography Includes bibliographical references and index
Notes English
Print version record
In Springer Protocols
Subject Chromatin -- Laboratory manuals
Telomere -- Laboratory manuals
ADP-ribosylation -- Laboratory manuals
Epigenesis -- Laboratory manuals
Chromatin Assembly and Disassembly -- genetics
Chromosome Structures -- metabolism
DNA Methylation
Epigenesis, Genetic
Histone Code -- genetics
Histones -- metabolism
SCIENCE -- Life Sciences -- Molecular Biology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Tollefsbol, Trygve O
LC no. 2004002870
ISBN 1592598285