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Title Telomeres and telomerase : methods and protocols / edited by John A. Double and Michael J. Thompson
Published Totowa, N.J. : Humana Press, ©2002


Description 1 online resource (xi, 221 pages) : illustrations
Series Methods in molecular biology ; v. 191
Methods in molecular biology (Clifton, N.J.) ; v. 191
Contents Introduction to telomeres and telomerase / Michael C. Bibby -- Detection of chromosome ends by telomere FISH / Harry Scherthan -- Telomere length distriubtion : digital image processing and statistical analysis / Jean-Patrick Pommier and Laure Sabatier -- Analysis of telomerase RNA gene expression by In Situ hybridization / W. Nicol Keith, Joseph Sarvesvaran and Martin Downey -- Relative gene expression in normal and tumor tissue by quantitative RT-PCR / Dennis S. Salonga, Kathleen D. Danenberg, Jean Grem, Ji Min Park and Peter V. Danenberg -- Quantitative detection of telomerase components by real-time, online RT-PCR analysis with the LightCycler / Thomas Emrich, Sheng-Yung Chang, Gerlinde Karl, Birgit Panzinger, and Chris Santini -- Standard TRAP assay / Angelika M. Burger -- Stretch PCR assay / Jun-ichi Nakayama and Fuyuki Ishikawa -- Fluorescent detection of telomerase activity / Wade K. Aldous, Amber J. Marean, Mary J. DeHart and Katherine H. Moore -- Nonradioactive detection of telomerase activity using a PCR-ELISA-based telomeric repeat amplification protocol / Thomas Emirch and Gerlinde Karl -- In Sute TRAP assay detection of telomerase activity in cytological preparations / Kazuma Ohyashiki and Junko H. Ohyashiki -- Biotinylated primer for detecting telomerase activity without amplification / Daekyu Sun -- Whole-cell and microcell fusion for the identification of natural regulators of telomerase / Henriette Gourdeau, Marsha D. Speevak, Lucie Jetté and Mario Chevrette -- Screening with COMPARE analysis for telomerase inhibitors / Imad Naasani, Takao Yamori and Takashi Tsuruo -- Telomerase as a therapeutic target : therapeutic potential of telomerase inhibitors / John A. Double
Summary The discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible protocols provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non-PCR-based methods to techniques using real-time PCR. Written by investigators who have used the methods extensively, each protocol includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Broad-ranging and highly practical, Telomeres and Telomerase: Methods and Protocols provides molecular and cell biologists, cancer researchers, and cellular pathologists with the core array of productive techniques needed today to develop telomerase inhibitors and clinically valuable diagnostic/prognostic telomerase markers
Bibliography Includes bibliographical references and index
Notes Print version record
In Springer Protocols
Subject Telomerase -- Laboratory manuals
Telomere -- Laboratory manuals
Telomere -- physiology
Telomerase -- physiology
SCIENCE -- Life Sciences -- Molecular Biology.
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Double, John A., editor.
Thompson, Michael J. (Michael Joseph), 1966- editor.
LC no. 2001039598
ISBN 9781592591893