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Title Telomeres and telomerase : methods and protocols / edited by Zhou Songyang
Edition Third edition
Published New York : Humana Press : Springer, [2017]
©2017

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Description 1 online resource (xi, 218 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1587
Methods in molecular biology (Clifton, N.J.) ; v. 1587.
Contents Introduction to telomeres and telomerase / Zhou Songyang -- Analysis of average telomere length in human telomeric protein knockout cells generated by CRISPR/Cas9 / Jun Xu, Zhou Songyang, Dan Liu, and Hyeung Kim -- Telomere length analysis by quantitative fluorescent in situ hybridization (Q-FISH) / Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo -- Telomere strand-specific length analysis by fluorescent in situ hybridization (Q-CO-FISH) / Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo -- Telomere G-rich overhang length measurement : DSN method / Yong Zhao, Jerry W. Shay, and Woodring E. Wright -- Telomere G-overhang length measurement method 2 : G-tail telomere HPA / Hidetoshi Tahara -- Telomere terminal G/C strand synthesis : measuring telomerase action and C-rich fill-in / Yong Zhao, Jerry W. Shay, and Woodring E. Wright -- Analysis of yeast telomerase by primer extension assays / Min Hsu and Neal F. Lue -- Assessing telomerase activities in mammalian cells using the quantitative PCR-based telomeric repeat amplification protocol (qTRAP) / Shuai Jiang, Mengfan Tang, Huawei Xin, and Junjiu Huang -- Telomeres and nextgen CO-FISH : directional genomic hybridization (Telo-dGH) / Miles J. McKenna, Erin Robinson, Edwin H. Goodwin, Michael N. Cornforth, and Susan M. Bailey -- Visualization of human telomerase localization by fluorescence microscopy techniques / Eladio Abreu, Rebecca M. Terns, and Michael P. Terns -- Cytogenetic analysis of telomere dysfunction / Rekha Rai, Asha S. Multani, and Sandy Chang -- Probing the telomere damage Response / Rekha Rai and Sandy Chang -- Induction of site-specific oxidative damage at telomeres by killerred-fused shelretin proteins / Rong Tan and Li Lan -- Using protein-fragment complementation assays (PCA) and peptide arrays to study telomeric protein-protein interactions / Wenbin Ma, Ok-hee Lee, Hyeung Kim, and Zhou Songyang -- In vitro preparation and crystallization of vertebrate telomerase subunits / Jing Huang, Christopher J. Bley, Dustin P. Rand, Julian J.L. Chen, and Ming Lei -- Human telomeric G-quadruplex structures and G-quadruplex-interactive compounds / Clement Lin and Danzhou Yang -- Analysis of telomere-homologous DNA with different conformations using 2D agarose electrophoresis and in-gel hybridization / Zepeng Zhang, Qian Hu, and Yong Zhao -- Analysis of telomere proteins by chromatin immunoprecipitation (ChIP) / Feng Liu, Xuyang Feng, and Wenbin Ma
Summary This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocols, viewed March 27, 2017)
Subject Telomerase -- Laboratory manuals
Telomere -- Laboratory manuals
Telomere.
Telomerase.
Telomere
Telomerase
Biochemistry.
Science -- Life Sciences -- Biochemistry.
Telomerase
Telomere
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Zhou, Songyang, editor
LC no. 2017932796
ISBN 9781493968923
1493968920