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Title Transcription factors : a practical approach / edited by David S. Latchman
Edition Second edition
Published Oxford ; New York : Oxford University Press, 1999
Online access available from:
EBSCO eBook Academic Collection    View Resource Record  


Description 1 online resource (xxii, 303 pages) : illustrations
Series The practical approach series ; 201
Practical approach series ; 201
Contents DNA mobility shift assay / C.L. Dent, M.D. Smith, D.S. Latchman -- Footprint analysis of DNA : protein complexes in vitro and in vivo / Craig Spiro, Cynthia T. McMurray -- In vitro transcription and characterization of transcription / Austin J. Cooney, Sophia Y. Tsai, Ming-Jer Tsai -- Purification and cloning of DNA binding transcription factors / R.H. Nicolas, G. Hynes, G.H. Goodwin -- Cloning transcription factors from a cDNA expression library / Ian G. Cowell, Helen C. Hurst -- Cloning transcription factors by sequence similarity / Alan Ashworth -- Identification of target genes for a transcription factor by genomic binding-site cloning / Satoshi Inoue, Shigeru Kondo, Masami Muramatsu -- Analysis of cloned factors / Roger White, Malcolm Parker -- Neuronal promoter analysis by in vitro transcription using nuclear extracts from rat brain / M.L. Schwartz, W.W. Schlaepfer -- Preparation of chromatin templates for transcription studies / Joan Boyes -- Analysis of transcription factor modifications / N. Shaun B. Thomas
Summary "Since publication of the first edition of this book five years ago, a wide range of new methodologies have been developed to facilitate studies on transcription factors. The book has been radically updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have either been fully updated or rewritten and additional chapters now included cover the use of in vitro transcription assays, analysis of the effect of transcription factors on chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications." "Each chapter includes full step-by-step protocols for immediate use at the laboratory bench and innumerable hints and tips for success. Anyone studying transcription factors in a research setting should find this book to be an invaluable bench companion." --Book Jacket
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Transcription factors -- Laboratory manuals.
Transcription Factors -- physiology.
Genre/Form Laboratory manuals.
Laboratory manuals.
Form Electronic book
Author Latchman, David S.
ISBN 0585483701 (electronic bk.)
9780585483702 (electronic bk.)