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Title Site-directed insertion of transgenes / Sylvaine Renault, Philippe Duchateau [editors]
Published Dordrecht ; New York : Springer, ©2013


Description 1 online resource
Series Topics in current genetics, 1610-2096 ; 23
Topics in current genetics ; 23.
Contents Transgene Site-Specific Integration: Problems and Solutions / Solenne Bire and Florence Rouleux-Bonnin -- Designing Non-viral Targeted Integrating Vectors for Genome Engineering in Vertebrates / Ludivine Sinzelle and Nicolas Pollet -- Gene Targeting and Homologous Recombination in Saccharomyces cerevisiae / Judith Miné-Hattab and Rodney Rothstein -- Homologous Recombination in Mammals / Aurélia Barascu, Anastazja Grabarz and Bernard S. Lopez -- Engineered Zinc Finger Nucleases for Targeted Genome Editing / Cherie L. Ramirez and J. Keith Joung -- Engineered Meganucleases for Genome Engineering Purposes / Jean Charles Epinat, George H. Silva, Frédéric Pâques, Julianne Smith and Philippe Duchateau -- Cre/loxP, Flp/FRT Systems and Pluripotent Stem Cell Lines / Candice G.T. Tahimic, Kenji Sakurai, Kazuhiro Aiba and Norio Nakatsuji -- Site-Specific Recombination Using PhiC31 Integrase / Jonathan M. Geisinger and Michele P. Calos -- Modified Transposases for Site-Directed Insertion of Transgenes / Sean Colloms and Sylvaine Renault -- Targeted Plasmid Integration into the Human Genome by Engineered Recombinases / Charles A. Gersbach and Carlos F. Barbas III -- Gene Site-Specific Insertion in Plants / Olivier Da Ines and Charles I. White -- Site-Directed Insertion of Transgenes in Drosophila / Dana Carroll -- Nuclease Mediated Targeted Genome Modification in Mammalian Cells / Jenny C. Barker, Richard A. Voit and Matthew H. Porteus -- Bio-applications Derived from Site-Directed Genome Modification Technologies / Christophe Delenda, Sébastien Paris, Sylvain Arnould, Edward Balbirnie and Jean-Pierre Cabaniols
Summary The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information's scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, jC31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information's will help students and experienced researchers to understand and choose the best technology for their own purposes
Analysis Chemistry
Gene expression
Gene therapy
Medical genetics
Genetic engineering
Gene Function
Bibliography Includes bibliographical references and index
In Springer eBooks
Subject Transgenes.
Genetic engineering.
Transgenes -- physiology
Gene Transfer Techniques
SCIENCE -- Biotechnology.
Genetic engineering
Form Electronic book
Author Renault, Sylvaine
Duchateau, Philippe
ISBN 9789400745315