| Description |
1 online resource (xiv, 294 pages, 67 illustrations, 50 illustrations in color.) |
| Series |
Methods in molecular biology, 1064-3745 ; 1659 |
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Methods in molecular biology ; 1659. 1064-3745
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| Contents |
Wheat rust surveillance : field disease scoring and sample collection for phenotyping and molecular genotyping / Sajid Ali, David Hodson -- Field pathogenomics : an advanced tool for wheat rust surveillance / Vanessa Bueno-Sancho, Daniel C.E. Bunting, Luis J. Yanes, Kentaro Yoshida, Diane G.O. Saunders -- Race typing of Puccinia striiformis on wheat / Mogens S. Hovmøller, Justin Rodríguez-Algaba, Tine Thach, Christ K. Sørensen -- Assessment of aggressiveness of Puccinia striiformis on wheat / Chris K. Sørensen, Tine Thach, Mogens S. Hovmøller -- Extraction of high molecular weight DNA from fungal rust spores for long read sequencing / Benjamin Schwessinger, John P. Rathjen -- Microsatellite genotyping of the wheat yellow rust pathogen Puccinia striiformis / Sajid Ali, Muhammed R. Khan, Angelique Gautier, Zahoor A. Swati, Stephanie Walter -- Computational methods for predicting effectors in rust pathogens / Jana Sperschneider, Peter N. Dodds, Jennifer M. Taylor, Sébastien Duplessis -- Protein-protein interaction assays with effector-GFP fusions in Nicotiana benthamiana / Benjamin Petre, Joe Win, Frank L.H. Menke, Sophien Kamoun -- Proteome profiling by 2D-liquid chromatography method for wheat-rust interaction / Semra Hasançebi -- Investigating gene fucntion in cereal rust fungi by plant-mediated virus-induced gene silencing / Vinay Panwar, Guus Bakkeren -- Apoplastic sugar extraction and quantification from wheat leaves infected with biotrophic fungi / Veronica Roman-Reyna, John P. Rathjen -- Genetic analysis of resistence to wheat rusts / Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, Ravi P. Singh -- Advances in identification and mapping of rust resistance genes in wheat / Urmil Bansal, Harbans Bariana Chromosome engineering techniques for targeted introgression of rust resistance from wild wheat relatives / Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, W. John Raupp -- Applications of genomic selection in breeding wheat for rust resistance / Leonardo Ornella, Juan Manuel González-Camacho, Susanne Dreisigacker, Jose Crossa -- Rapid phenotying adult plant resistance to stem rust in wheat grown under controlled conditions / Adnan Riaz, Lee T. Hickey -- Generation of loss-of-function mutants for wheat rust disease resistance gene cloning / Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, Evans Lagudah -- Isolation of wheat genomic DNA for gene mapping and cloning / Guotai Yu, Asyraf Hatta, Sambasivam Periyannan, Evans Lagudah, Brande B.H. Wulff -- MutRenSeq : a method for rapid cloning of plant disease resistance genes / Burkhard Steuernagel, Kamil Witek, Jonathan D.G. Jones, Brande B.H. Wulff -- Rapid gene isolation using MutChromSeq / Burkhard Steuernagel, Jan Vrána, Miroslava Karafiátová, Brande B.H. Wulff, Jaroslav Doležel -- Rapid identification of rust resistance genes through cultivar-specific De novo chromosome assemblies / Anupriya K. Thind, Thomas Wicker, Simon G. Krattinger -- BSMV-induced gene silencing assay for functional analysis of wheat rust resistance / Li Huang -- Yeast as a heterologous system to functionally characterize a multiple rust resistance gene that encodes a hexose transporter / Ricky J. Milne, Katherine E. Dibley, Evans S. Lagudah -- Biocontrol agents for controlling wheat rust / Siliang Huang, Fahu Pang |
| Summary |
This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Life sciences.
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Botany.
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Triticum -- microbiology
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Biological Science Disciplines
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biological sciences.
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Botany & plant sciences.
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Science -- Life Sciences -- Botany.
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Botany
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Life sciences
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Periyannan, Sambasivam, editor
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| ISBN |
9781493972494 |
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1493972499 |
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