| Description |
1 online resource (1 volume) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 361, pt. 2 |
|
Methods in molecular biology (Clifton, N.J.) ; volume 361, pt. 2
|
| Contents |
Main Approaches to Target Discovery and Validation / Mouldy Sioud -- Bioinformatics Approaches to Cancer Gene Discovery / Ramaswamy Narayanan -- Analysis of Gene Networks for Drug Target Discovery and Validation / Seiya Imoto [and others] -- Target Discovery and Validation in Pancreatic Cancer / Robert M. Beaty [and others] -- Molecular Classification of Breast Tumors: Toward Improved Diagnostics and Treatments / Therese Sørlie -- Discovery of Differentially Expressed Genes: Technical Considerations / Øystein Røsok and Mouldy Sioud -- Genome-Wide Screening by Using Small-Interfering RNA-Expression Libraries / Sahohime Matsumoto, Makoto Miyagishi, and Kazunari Taira -- Hammerhead Ribozyme-Based Target Discovery / Masayuki Sano and Kazunari Taira -- Production of siRNA and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells / Holger Erfle and Rainer Pepperkok -- Transgenic Animal Models in Biomedical Research / Louis-Marie Houdebine -- Keratin Trangenic and Knockout Mice: Functional Analysis and Validation of Disease-Causing Mutations / Preethi Vijayaraj, Goran Söhl, and Thomas M. Magin -- The HUVEC/Matrigel Assay: An In Vivo Assay of Human Angiogenesis Suitable for Drug Validation / Dag K. Skovseth, Axel M. Küchler, and Guttorm Haraldsen -- A Murine Model for Studying Hematopoiesis and Immunity in Heart Failure / Per Ole Iversen and Dag R. Sørensen -- An Overview of the Immune System and Technical Advances in Tumor Antigen Discovery and Validation / Mouldy Sioud -- Potential Target Antigens for Immunotherapy Identified by Serological Expression Cloning (SEREX) / Dirk Jäger -- Identification of Tumor Antigens by Using Proteomics / Francois Le Naour -- Protein Arrays: A Versatile Toolbox for Target Identification and Monitoring of Patient Immune Responses / Lina Cekaite, Eivind Hovig, and Mouldy Sioud -- Index |
| Summary |
Target Discovery and Validation Reviews and Protocols, Volumes 1 and 2 review the most progressive and current methods for drug target discovery and validation. These volumes explore how recent improvement in understanding the molecular mechanisms of human pathology is impacting drug target discovery in the laboratory and in real therapeutics, specifically for cancers and autoimmune disorders. Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date protocols available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. The profound impact of genomics, proteomics and bioinformatics on target discovery is explored, and specific attention is given to the role of transgenic and knockout animals in functional genomics and target validation. Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of protocols for tumor antigens and cancer vaccines. The methods and protocols collected here, all reviewed by leading scientists and clinicians, present the practical details necessary for translating the enormous discovery potential of the genome into real therapeutic products. Volume 2 collects all the practical details required for efficient translation of discovered targets into real pharmaceutical drugs. Specific targets in cancers and autoimmunity are described and the potential of using siRNAs, antisense oligonucleotides and RNA aptamers in patients is reviewed. This volume explores the tremendous impact of the application of genotyping and gene expression profiling on the future of healthcare, and presents cutting-edge protocols to aid in bringing agents against specific targets closer to application in the clinic. Collectively, these volumes provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application. For researchers, an understanding of available methods aids in the creation of innovative experiments in the laboratory, and the successful translation of target discovery to real therapeutics |
| Analysis |
neoplasms |
|
geneeskunde |
|
medicine |
|
geneesmiddelen |
|
drugs |
|
geneesmiddelenbehandeling |
|
drug therapy |
|
biologische technieken |
|
biological techniques |
|
gene discovery |
|
protocollen |
|
protocols |
|
Molecular Biology (General) |
|
Medicine (General) |
|
Moleculaire biologie (algemeen) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Print version record |
| Subject |
Tumor markers.
|
|
Biochemical markers -- Therapeutic use
|
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DNA microarrays.
|
|
Drug targeting.
|
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Drugs -- Design.
|
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Molecular pharmacology.
|
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Antineoplastic agents.
|
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Antimitotic agents.
|
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Antineoplastic Agents
|
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Drug Delivery Systems
|
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Drug Design
|
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Biomarkers, Tumor
|
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Antimitotic Agents
|
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MEDICAL -- Oncology.
|
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HEALTH & FITNESS -- Diseases -- Cancer.
|
|
DNA microarrays.
|
|
Drug targeting.
|
|
Drugs -- Design.
|
|
Molecular pharmacology.
|
|
Antineoplastic agents.
|
|
Drug Design.
|
|
Tumor Markers, Biological.
|
|
Tumor markers.
|
|
Biochemical markers -- Therapeutic use.
|
|
Antineoplastic agents
|
|
Antimitotic agents
|
|
DNA microarrays
|
|
Drug targeting
|
|
Drugs -- Design
|
|
Molecular pharmacology
|
|
Tumor markers
|
|
Neoplasias.
|
|
Antineoplásicos.
|
|
Química farmacêutica.
|
|
Farmacologia molecular.
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Sioud, Mouldy.
|
| ISBN |
9781597452083 |
|
1597452084 |
|
9781588298904 |
|
1588298906 |
|
