| Description |
1 online resource (xi, 279 pages) : illustrations |
| Series |
Methods in molecular medicine, 1543-1894 ; 98 |
|
Methods in molecular medicine ; 98. 1543-1894
|
| Contents |
Tumor necrosis factor as a pharmacological target / Pietro Ghezzi and Antony Cerami -- Production and characterization of recombinant human and murine TNF / Flavio Curnis and Angelo Corti -- Purification of TNF binding proteins / Hartmut Engelmann, Dani Aderka, and David Wallach -- Production and characterization of receptor specific TNF muteins / Paul Ameloot and Peter Brouckaert -- TNF promoter polymorphysms / Silvia Fargion [and others] -- Detection of TNF and TNF receptor mRNA in cells and tissues / Roberto Furlan [and others] -- Immuno-histochemical localization of TNF and its receptors / Paolo Bigini and Tiziana Mennini -- TNF signaling: key protocols / Marta Muzio and Simona Saccani -- More than one way to die: methods to determine TNF-induced apoptosis and necrosis / Tom Vanden Berghe [and others] -- Assessment of the endothelial TNF-induced damage through the loss of its barrier function / Elisabetta Ferrero |
|
Genetic engineering in the mouse: tuning TNF/TNF-R expression / Eleni Douni, Maria Alexiou, and George Kollias -- Animal models of TNF-mediated diseases of the central nervous system -- Roberto Furlan [and others] -- In vivo vascular leakage assay / Maria Elena Ferrero -- Animal models of endotoxic shock / Pia Villa and Pietro Ghezzi -- Collagen-induced arthritis as a model for rheumatoid arthritis / Richard O. Williams -- Miocardial infarction: animal models / Stefano Chimenti [and others] -- Tumor vascular therapy with TNF: critical review on animal models / Timo L.M. ten Hagen and Alexander M.M. Eggermont -- Strategies for improving the anti-neoplastic activity of TNF by tumor targeting / Angelo Corti |
| Summary |
A comprehensive collection of readily reproducible techniques for TNF production, characterization, mutagenesis, and detection in biological specimens. Also included are several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The collection offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of versatile, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| In |
Springer Protocols |
| Subject |
Tumor necrosis factor -- Laboratory manuals
|
|
Tumor necrosis factor.
|
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Diseases -- Animal models.
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Tumor necrosis factor -- Receptors.
|
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Tumor Necrosis Factor-alpha
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Disease Models, Animal
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Receptors, Tumor Necrosis Factor
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MEDICAL -- Immunology.
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Tumor necrosis factor -- Receptors
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Diseases -- Animal models
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Tumor necrosis factor
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| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Corti, Angelo
|
|
Ghezzi, P. (Pietro)
|
| ISBN |
9781592597710 |
|
1592597718 |
|
1588292231 |
|
9781588292230 |
|
1280360305 |
|
9781280360305 |
|
9786610360307 |
|
6610360308 |
|
