Title Cancer cell culture : methods and protocols / edited by Simon P. Langdon

Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xii, 360 pages) : illustrations Series Methods in molecular medicine, 1543-1894 ; 88 Methods in molecular medicine ; 88. 1543-1894 Contents Basic principles of cancer cell culture / S.P. Langdon -- Essential techniques of cancer cell culture / K.G. MacLeod and S.P. Langdon -- Characterization and authentication of cancer cell lines: an overview / S.P. Langdon -- Authentication of cancer cell lines by DNA fingerprinting / W.G. Dirks and H.G. Drexler -- Cytogenetic characterization of tumor cell lines / R.A. MacLeod and H.G. Drexler -- Isolation and culture of colon cancer cell lines / J.G. Park, J.L. Ku and S.Y. Park -- Isolation and culture of melanoma cell lines / A.I. Riker -- Isolation and culture of human brain tumor cells / G.G. Gomez and C.A. Kruse -- Isolation and culture of renal cancer cell lines / J.G. Park, J.L. Ku and S.Y. Park -- Isolation and culture of prostate cancer cell lines / N.M. Navone, M. Olive and P. Troncoso -- Isolation and culture of ovarian cancer cell lines / S.P. Langdon -- Isolation and culture of leukemia cell lines / H.G. Drexler -- Cell sensitivity assays: clonogenic assay / J.A. Plumb -- Cell sensitivity assays: the MTT assay / J.A. Plumb -- PARP cleavage as a means of assessing apoptosis / P. Mullen -- Detection of apoptosis by the TUNEL assay / J. Lawry -- Apoptosis measurement by annexin v staining / E. Miller -- Cell adhesion assays / W.N. Scott -- Radial monolayer cell migration assay / M.E. Berens and C. Beaudry -- Invasion and motility assays / W.N. Scott -- Identification of senescence in cancer cells / L.J. Jardine -- Angiogenesis assays / W.N. Scott -- Flow cytometric DNA analysis of human cancer cell lines / P. Mullen -- DNA-mediated gene transfer / A.J. O'Donnell -- Development of drug-resistant models / H.M. Coley -- Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene / N.J. Maitland [and others] -- The use of Matrigel to facilitate the establishment of human cancer cell lines as xenografts / P. Mullen -- Coculture of prostate cancer cells / S.H. Lang and N.J. Maitland -- Coculture of ovarian cells using porous tissue culture inserts / K.E. Snow and S.P. Langdon -- Cell culture contamination: an overview / S.P. Langdon -- Detecting Mycoplasma contamination in cell cultures by polymerase chain reaction / C.C. Uphoff and H.G. Drexler -- Elimination of Mycoplasma from infected cell lines using antibiotics / C.C. Uphoff and H.G. Drexler Summary The growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. Suitable for novice and experienced researchers alike, these readily reproducible techniques solve a diverse range of experimental problems. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the coculture of different cell types; and the detection and treatment of contamination. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cancer Cell Culture: Methods and Protocols provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Cancer cells -- Laboratory manuals Tumor Cells, Cultured Laboratory Techniques and Procedures MEDICAL -- Oncology. HEALTH & FITNESS -- Diseases -- Cancer. Cancer cells Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Langdon, Simon P. ISBN 9781592594061 1588290794 9781588290793 1592594069 1280842792 9781280842795 9786610842797 6610842795

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